Table of Contents

October 2006; 2006 (5)

Protocols

  • Staining Membrane-Bound Proteins with Ponceau S

    • Wayne R. Stochaj,
    • Tom Berkelman,
    • and Nancy Laird
    Cold Spring Harb Protoc; 2006; doi:10.1101/pdb.prot4543

  • Staining Membrane-Bound Proteins with Coomassie Blue R250

    • Wayne R. Stochaj,
    • Tom Berkelman,
    • and Nancy Laird
    Cold Spring Harb Protoc; 2006; doi:10.1101/pdb.prot4544

  • Staining Membrane-Bound Proteins with Colloidal Gold

    • Wayne R. Stochaj,
    • Tom Berkelman,
    • and Nancy Laird
    Cold Spring Harb Protoc; 2006; doi:10.1101/pdb.prot4545

  • Packing Capillary Columns for RP-HPLC

    • Robert Moritz
    Cold Spring Harb Protoc; 2006; doi:10.1101/pdb.prot4546

  • Purification of Large “Troublesome” Polypeptides by RP-HPLC

    • Hooi Hong Keah and
    • Milton T.W. Hearn
    Cold Spring Harb Protoc; 2006; doi:10.1101/pdb.prot4547

  • Purification of Peptides from Solid-Phase Peptide Synthesis with RP-HPLC

    • Reinhard I. Boysen and
    • Milton T.W. Hearn
    Cold Spring Harb Protoc; 2006; doi:10.1101/pdb.prot4548

  • Desalting of Peptides and Protein Mixtures by RP-HPLC Techniques

    • Reinhard I. Boysen and
    • Milton T.W. Hearn
    Cold Spring Harb Protoc; 2006; doi:10.1101/pdb.prot4549

  • Fragmentation of Protein Using Trypsin

    • Richard J. Simpson
    Cold Spring Harb Protoc; 2006; doi:10.1101/pdb.prot4550

  • Modification of Lysyl Side Chains Using Succinic Anhydride

    • Richard J. Simpson
    Cold Spring Harb Protoc; 2006; doi:10.1101/pdb.prot4551

  • Modification of Lysyl Side Chains Using Citraconic Anhydride

    • Richard J. Simpson
    Cold Spring Harb Protoc; 2006; doi:10.1101/pdb.prot4552

  • Analysis of Complex Protein Mixtures Using Nano-LC Coupled to MS/MS

    • David M. Schieltz,
    • Michael P. Washburn,
    • and Lara G. Hays
    Cold Spring Harb Protoc; 2006; doi:10.1101/pdb.prot4553

  • Tandem Mass Spectrometry Analysis Using the ThermoFinnigan LCQ System

    • David M. Schieltz,
    • Michael P. Washburn,
    • and Lara G. Hays
    Cold Spring Harb Protoc; 2006; doi:10.1101/pdb.prot4554

  • Analysis of Complex Protein Mixtures Using Multidimensional Protein Identification Technology (MuDPIT)

    • David M. Schieltz and
    • Michael P. Washburn
    Cold Spring Harb Protoc; 2006; doi:10.1101/pdb.prot4555

  • Digestion of Insoluble Protein Fractions for Multidimensional Protein Identification Technology (MuDPIT) Analysis

    • David M. Schieltz and
    • Michael P. Washburn
    Cold Spring Harb Protoc; 2006; doi:10.1101/pdb.prot4556

  • Using FLAG Epitope-Tagged Proteins for Coimmunoprecipitation of Interacting Proteins

    • Anne Verhagen
    Cold Spring Harb Protoc; 2006; doi:10.1101/pdb.prot4557

  • Preparative 2D Gel Electrophoresis with Immobilized pH Gradients: SDS-PAGE of Proteins

    • Wayne R. Stochaj,
    • Tom Berkelman,
    • and Nancy Laird
    Cold Spring Harb Protoc; 2006; doi:10.1101/pdb.prot4559

  • Fluorescent Staining of Proteins with SYPRO Ruby

    • Richard J. Simpson
    Cold Spring Harb Protoc; 2006; doi:10.1101/pdb.prot4567

  • Preparation of Eukaryotic Lysates for 2D Gel Electrophoresis

    • Hong Ji
    Cold Spring Harb Protoc; 2006; doi:10.1101/pdb.prot4571

  • Radioisotopic Labeling of Eukaryotic Cell Proteins Using 35S

    • Hong Ji
    Cold Spring Harb Protoc; 2006; doi:10.1101/pdb.prot4572

  • Radioisotopic Labeling of Eukaryotic Cell Proteins Using 32P

    • Hong Ji
    Cold Spring Harb Protoc; 2006; doi:10.1101/pdb.prot4573

  • Preparative 2D Gel Electrophoresis with Immobilized pH Gradients: Rehydration of IPG Strips for Isoelectric Focusing of Proteins

    • Wayne R. Stochaj,
    • Tom Berkelman,
    • and Nancy Laird
    Cold Spring Harb Protoc; 2006; doi:10.1101/pdb.prot4574

  • Preparative 2D Gel Electrophoresis with Immobilized pH Gradients: Isoelectric Focusing of Proteins in a Multipurpose Flatbed Electrophoresis Unit

    • Wayne R. Stochaj,
    • Tom Berkelman,
    • and Nancy Laird
    Cold Spring Harb Protoc; 2006; doi:10.1101/pdb.prot4575

  • Preparative 2D Gel Electrophoresis with Immobilized pH Gradients: IEF of Proteins in an IEF-Dedicated Electrophoresis Unit

    • Wayne R. Stochaj,
    • Tom Berkelman,
    • and Nancy Laird
    Cold Spring Harb Protoc; 2006; doi:10.1101/pdb.prot4576

  • Phosphoprotein Staining with the GelCode Phosphoprotein Staining Kit

    • Wayne R. Stochaj,
    • Tom Berkelman,
    • and Nancy Laird
    Cold Spring Harb Protoc; 2006; doi:10.1101/pdb.prot4577

  • Analysis of Protein Interactions In Vivo with Fluorescence Resonance Energy Transfer (FRET)

    • Marc Damelin and
    • Pamela Silver
    Cold Spring Harb Protoc; 2006; doi:10.1101/pdb.prot4581

  • Preparation of Vertical SDS Slab Gels: Casting a Single Homogeneous Gel

    • Wayne R. Stochaj,
    • Tom Berkelman,
    • and Nancy Laird
    Cold Spring Harb Protoc; 2006; doi:10.1101/pdb.prot4582

  • Preparation of Vertical SDS Slab Gels: Simultaneous Casting of Multiple-Gradient Gels

    • Wayne R. Stochaj,
    • Tom Berkelman,
    • and Nancy Laird
    Cold Spring Harb Protoc; 2006; doi:10.1101/pdb.prot4583

  • Preparative 2D Gel Electrophoresis with Immobilized pH Gradients: IPG Strip Equilibration

    • Wayne R. Stochaj,
    • Tom Berkelman,
    • and Nancy Laird
    Cold Spring Harb Protoc; 2006; doi:10.1101/pdb.prot4584

  • Concentrating Acrylamide Gel Spots

    • Richard J. Simpson
    Cold Spring Harb Protoc; 2006; doi:10.1101/pdb.prot4585

  • Gateway-Compatible Yeast One-Hybrid Screens

    • Bart Deplancke,
    • Vanessa Vermeirssen,
    • H. Efsun Arda,
    • Natalia J. Martinez,
    • and Albertha J.M. Walhout
    Cold Spring Harb Protoc; 2006; doi:10.1101/pdb.prot4590

  • EMS Mutagenesis of Arabidopsis Seed

    • Detlef Weigel and
    • Jane Glazebrook
    Cold Spring Harb Protoc; 2006; doi:10.1101/pdb.prot4621

  • Screening DNA Pools for T-DNA Insertions in Arabidopsis Genes

    • Detlef Weigel and
    • Jane Glazebrook
    Cold Spring Harb Protoc; 2006; doi:10.1101/pdb.prot4622

  • Setting Up Arabidopsis Crosses

    • Detlef Weigel and
    • Jane Glazebrook
    Cold Spring Harb Protoc; 2006; doi:10.1101/pdb.prot4623

Information Panel

  • Chromatographic Conditions for the Analytical Separation of Crude Peptide and Protein Mixtures

    • Reinhard I. Boysen and
    • Milton T.W. Hearn
    Cold Spring Harb Protoc; 2006; doi:10.1101/pdb.ip3

  • Chromatographic Conditions for the Preparative Separation of Peptides

    • Reinhard I. Boysen and
    • Milton T.W. Hearn
    Cold Spring Harb Protoc; 2006; doi:10.1101/pdb.ip4

  • The Use of Mass Spectrometry in Proteomics: Database Searching

    • David M. Schieltz,
    • Michael P. Washburn,
    • and Lara G. Hays
    Cold Spring Harb Protoc; 2006; doi:10.1101/pdb.ip5

  • Chromatographic Conditions for the Desalting of Peptides and Proteins

    • Reinhard I. Boysen and
    • Milton T.W. Hearn
    Cold Spring Harb Protoc; 2006; doi:10.1101/pdb.ip6

  • Equipment for In Vivo FRET Analysis

    • Marc Damelin and
    • Pamela Silver
    Cold Spring Harb Protoc; 2006; doi:10.1101/pdb.ip8

  • An Explanation of Symbols Used for Analysis of FRET Data

    • Marc Damelin and
    • Pamela Silver
    Cold Spring Harb Protoc; 2006; doi:10.1101/pdb.ip9

  • Experimental Design for In Vivo FRET Analysis

    • Marc Damelin and
    • Pamela Silver
    Cold Spring Harb Protoc; 2006; doi:10.1101/pdb.ip10

  • Cultivation of Arabidopsis

    • Daphne Preuss
    Cold Spring Harb Protoc; 2006; doi:10.1101/pdb.ip22

Topic Introduction

  • Forward Genetics in Arabidopsis: Finding Mutations that Cause Particular Phenotypes

    • Detlef Weigel and
    • Jane Glazebrook
    Cold Spring Harb Protoc; 2006; doi:10.1101/pdb.top1
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October 2006; 2006 (5)
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