Table of Contents

Protocols

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  Protein Interactions Captured by Chemical Cross-linking: Simple Cross-linking Screen Using Sulfo-MBS

  • Owen W. Nadeau and
  • Gerald M. Carlson

  Cold Spring Harb Protoc; 2007; doi:10.1101/pdb.prot4587
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  Protein Interactions Captured by Chemical Cross-linking: One-Step Cross-linking with Formaldehyde

  • Owen W. Nadeau and
  • Gerald M. Carlson

  Cold Spring Harb Protoc; 2007; doi:10.1101/pdb.prot4634
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  Protein Interactions Captured by Chemical Cross-linking: Two-Step Cross-linking with ANB•NOS

  • Owen W. Nadeau and
  • Gerald M. Carlson

  Cold Spring Harb Protoc; 2007; doi:10.1101/pdb.prot4635
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  Using Genetically Engineered Kinases to Screen for Novel Protein Kinase Substrates: Identification of a Mutant Kinase/ATP Analog Pair

  • Scott T. Eblen,
  • N. Vinay Kumar,
  • and Michael J. Weber

  Cold Spring Harb Protoc; 2007; doi:10.1101/pdb.prot4636
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  Using Genetically Engineered Kinases to Screen for Novel Protein Kinase Substrates: Generation of [γ-³²P]ATP Analog from ADP Analog

  • Scott T. Eblen,
  • N. Vinay Kumar,
  • and Michael J. Weber

  Cold Spring Harb Protoc; 2007; doi:10.1101/pdb.prot4637
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  Using Genetically Engineered Kinases to Screen for Novel Protein Kinase Substrates: Phosphorylation of Kinase-Associated Substrates

  • Scott T. Eblen,
  • N. Vinay Kumar,
  • and Michael J. Weber

  Cold Spring Harb Protoc; 2007; doi:10.1101/pdb.prot4638
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  Using Genetically Engineered Kinases to Screen for Novel Protein Kinase Substrates: Phosphorylation of Substrates in Cell Lysates with Exogenous Kinase

  • Scott T. Eblen,
  • N. Vinay Kumar,
  • and Michael J. Weber

  Cold Spring Harb Protoc; 2007; doi:10.1101/pdb.prot4639
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  Screening Kinase Phosphorylation Motifs Using Peptide Libraries

  • Isaac A. Manke and
  • Michael B. Yaffe

  Cold Spring Harb Protoc; 2007; doi:10.1101/pdb.prot4640
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  Zinc/Imidazole Procedure for Visualization of Proteins in Gels by Negative Staining

  • Richard J. Simpson

  Cold Spring Harb Protoc; 2007; doi:10.1101/pdb.prot4701
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  Dissection Techniques for Pupal and Larval Drosophila Eyes

  • Tanya Wolff

  Cold Spring Harb Protoc; 2007; doi:10.1101/pdb.prot4715
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  Staining of Drosophila Tissues with Antibodies

  • Tanya Wolff

  Cold Spring Harb Protoc; 2007; doi:10.1101/pdb.prot4716
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  Collection, Dechorionation, and Preparation of Drosophila Embryos for Quantitative Microinjection

  • Daniel P. Kiehart,
  • Janice M. Crawford,
  • and Ruth A. Montague

  Cold Spring Harb Protoc; 2007; doi:10.1101/pdb.prot4717
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  Quantitative Microinjection of Drosophila Embryos

  • Daniel P. Kiehart,
  • Janice M. Crawford,
  • and Ruth A. Montague

  Cold Spring Harb Protoc; 2007; doi:10.1101/pdb.prot4718
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  Staining Proteins in Gels with Coomassie Blue

  • Richard J. Simpson

  Cold Spring Harb Protoc; 2007; doi:10.1101/pdb.prot4719
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  Imaging X-gal-Stained Mouse Embryos

  • Akio Kobayashi

  Cold Spring Harb Protoc; 2007; doi:10.1101/pdb.prot4724
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  Staining Whole Mouse Embryos for β-Galactosidase (lacZ) Activity

  • Andras Nagy,
  • Marina Gertsenstein,
  • Kristina Vintersten,
  • and Richard Behringer

  Cold Spring Harb Protoc; 2007; doi:10.1101/pdb.prot4725
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  Staining Frozen Mouse Embryo Sections for β-Galactosidase (lacZ) Activity

  • Andras Nagy,
  • Marina Gertsenstein,
  • Kristina Vintersten,
  • and Richard Behringer

  Cold Spring Harb Protoc; 2007; doi:10.1101/pdb.prot4726
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  Preparation of GST Fusion Proteins

  • Margret B. Einarson,
  • Elena N. Pugacheva,
  • and Jason R. Orlinick

  Cold Spring Harb Protoc; 2007; doi:10.1101/pdb.prot4738
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  cAMP Assays

  • Gouzel Karimova and
  • Daniel Ladant

  Cold Spring Harb Protoc; 2007; doi:10.1101/pdb.prot4739
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