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High-Throughput Cloning of Open Reading Frames (ORFs): Assembling Large Sets of Expression Constructs

Andrew J. Link and
Joshua LaBaer

Cold Spring Harb Protoc; 2008; doi:10.1101/pdb.prot5050

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Cultivating Antirrhinum

Andrew Hudson,
Joanna Critchley,
and Yvette Erasmus

Cold Spring Harb Protoc; 2008; doi:10.1101/pdb.prot5051

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Propagating Antirrhinum

Andrew Hudson,
Joanna Critchley,
and Yvette Erasmus

Cold Spring Harb Protoc; 2008; doi:10.1101/pdb.prot5052

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Establishing and Maintaining a Colony of Planarians

Néstor J. Oviedo,
Cindy L. Nicolas,
Dany S. Adams,
and Michael Levin

Cold Spring Harb Protoc; 2008; doi:10.1101/pdb.prot5053

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Gene Knockdown in Planarians Using RNA Interference

Néstor J. Oviedo,
Cindy L. Nicolas,
Dany S. Adams,
and Michael Levin

Cold Spring Harb Protoc; 2008; doi:10.1101/pdb.prot5054

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Live Imaging of Planarian Membrane Potential Using DiBAC₄(3)

Néstor J. Oviedo,
Cindy L. Nicolas,
Dany S. Adams,
and Michael Levin

Cold Spring Harb Protoc; 2008; doi:10.1101/pdb.prot5055

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Isolation of Pristionchus Nematodes from Beetles

Robbie Rae,
Benjamin Schlager,
and Ralf J. Sommer

Cold Spring Harb Protoc; 2008; doi:10.1101/pdb.prot5063

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Assessment of the Olfactory Response to Chemicals or Bacteria in Pristionchus Nematodes

Robbie Rae,
Benjamin Schlager,
and Ralf J. Sommer

Cold Spring Harb Protoc; 2008; doi:10.1101/pdb.prot5064

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Isolation of RNA and DNA from Biofilm Samples Obtained by Laser Capture Microdissection Microscopy

Ailyn C. Pérez-Osorio and
Michael J. Franklin

Cold Spring Harb Protoc; 2008; doi:10.1101/pdb.prot5065

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qRT-PCR of Microbial Biofilms

Ailyn C. Pérez-Osorio and
Michael J. Franklin

Cold Spring Harb Protoc; 2008; doi:10.1101/pdb.prot5066

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Collection and Fixation of Spider Embryos

Nikola-Michael Prpic,
Michael Schoppmeier,
and Wim G.M. Damen

Cold Spring Harb Protoc; 2008; doi:10.1101/pdb.prot5067

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Whole-Mount In Situ Hybridization of Spider Embryos

Nikola-Michael Prpic,
Michael Schoppmeier,
and Wim G.M. Damen

Cold Spring Harb Protoc; 2008; doi:10.1101/pdb.prot5068

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Detection of Cell Death in Spider Embryos Using TUNEL

Nikola-Michael Prpic,
Michael Schoppmeier,
and Wim G.M. Damen

Cold Spring Harb Protoc; 2008; doi:10.1101/pdb.prot5069

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Gene Silencing via Embryonic RNAi in Spider Embryos

Nikola-Michael Prpic,
Michael Schoppmeier,
and Wim G.M. Damen

Cold Spring Harb Protoc; 2008; doi:10.1101/pdb.prot5070

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Detection of Cell Proliferation in Spider Embryos Using BrdU Labeling

Nikola-Michael Prpic,
Michael Schoppmeier,
and Wim G.M. Damen

Cold Spring Harb Protoc; 2008; doi:10.1101/pdb.prot5071

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Dissecting Spider Embryos for Light Microscopy

Nikola-Michael Prpic,
Michael Schoppmeier,
and Wim G.M. Damen

Cold Spring Harb Protoc; 2008; doi:10.1101/pdb.prot5072

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Basic Maintenance and Breeding of the Opossum Monodelphis domestica

Anna L. Keyte and
Kathleen K. Smith

Cold Spring Harb Protoc; 2008; doi:10.1101/pdb.prot5073

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Harvesting Monodelphis Embryos

Anna L. Keyte and
Kathleen K. Smith

Cold Spring Harb Protoc; 2008; doi:10.1101/pdb.prot5074

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Monodelphis Whole-Embryo Culture

Anna L. Keyte and
Kathleen K. Smith

Cold Spring Harb Protoc; 2008; doi:10.1101/pdb.prot5075

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Whole-Mount In Situ Hybridization in Monodelphis Embryos

Anna L. Keyte and
Kathleen K. Smith

Cold Spring Harb Protoc; 2008; doi:10.1101/pdb.prot5076

Emerging Model Organisms

The Genus Antirrhinum (Snapdragon): A Flowering Plant Model for Evolution and Development

Planarians: A Versatile and Powerful Model System for Molecular Studies of Regeneration, Adult Stem Cell Regulation, Aging, and Behavior

Pristionchus pacificus: A Genetic Model System for the Study of Evolutionary Developmental Biology and the Evolution of Complex Life-History Traits

The American Wandering Spider Cupiennius salei

Opossum (Monodelphis domestica): A Marsupial Development Model

Protocols

High-Throughput Cloning of Open Reading Frames (ORFs): Assembling Large Sets of Expression Constructs

Cultivating Antirrhinum

Propagating Antirrhinum

Establishing and Maintaining a Colony of Planarians

Gene Knockdown in Planarians Using RNA Interference

Live Imaging of Planarian Membrane Potential Using DiBAC₄(3)

Isolation of Pristionchus Nematodes from Beetles

Assessment of the Olfactory Response to Chemicals or Bacteria in Pristionchus Nematodes

Isolation of RNA and DNA from Biofilm Samples Obtained by Laser Capture Microdissection Microscopy

qRT-PCR of Microbial Biofilms

Collection and Fixation of Spider Embryos

Whole-Mount In Situ Hybridization of Spider Embryos

Detection of Cell Death in Spider Embryos Using TUNEL

Gene Silencing via Embryonic RNAi in Spider Embryos

Detection of Cell Proliferation in Spider Embryos Using BrdU Labeling

Dissecting Spider Embryos for Light Microscopy

Basic Maintenance and Breeding of the Opossum Monodelphis domestica

Harvesting Monodelphis Embryos

Monodelphis Whole-Embryo Culture

Whole-Mount In Situ Hybridization in Monodelphis Embryos

This Issue

Current Issue

From the cover

Table of Contents

Emerging Model Organisms

The Genus Antirrhinum (Snapdragon): A Flowering Plant Model for Evolution and Development

Planarians: A Versatile and Powerful Model System for Molecular Studies of Regeneration, Adult Stem Cell Regulation, Aging, and Behavior

Pristionchus pacificus: A Genetic Model System for the Study of Evolutionary Developmental Biology and the Evolution of Complex Life-History Traits

The American Wandering Spider Cupiennius salei

Opossum (Monodelphis domestica): A Marsupial Development Model

Protocols

High-Throughput Cloning of Open Reading Frames (ORFs): Assembling Large Sets of Expression Constructs

Cultivating Antirrhinum

Propagating Antirrhinum

Establishing and Maintaining a Colony of Planarians

Gene Knockdown in Planarians Using RNA Interference

Live Imaging of Planarian Membrane Potential Using DiBAC4(3)

Isolation of Pristionchus Nematodes from Beetles

Assessment of the Olfactory Response to Chemicals or Bacteria in Pristionchus Nematodes

Isolation of RNA and DNA from Biofilm Samples Obtained by Laser Capture Microdissection Microscopy

qRT-PCR of Microbial Biofilms

Collection and Fixation of Spider Embryos

Whole-Mount In Situ Hybridization of Spider Embryos

Detection of Cell Death in Spider Embryos Using TUNEL

Gene Silencing via Embryonic RNAi in Spider Embryos

Detection of Cell Proliferation in Spider Embryos Using BrdU Labeling

Dissecting Spider Embryos for Light Microscopy

Basic Maintenance and Breeding of the Opossum Monodelphis domestica

Harvesting Monodelphis Embryos

Monodelphis Whole-Embryo Culture

Whole-Mount In Situ Hybridization in Monodelphis Embryos

This Issue

Current Issue

From the cover

Live Imaging of Planarian Membrane Potential Using DiBAC₄(3)