Table of Contents

Protocols

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  PCR Mutagenesis by Overlap Extension and Gene SOE

  • Abbe N. Vallejo,
  • Robert J. Pogulis,
  • and Larry R. Pease

  Cold Spring Harb Protoc; 2008; doi:10.1101/pdb.prot4861
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  Preparation of Double-Stranded RNA for Drosophila RNA Interference (RNAi)

  • Leonie Misquitta,
  • Qin Wei,
  • and Bruce M. Paterson

  Cold Spring Harb Protoc; 2008; doi:10.1101/pdb.prot4916
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  Collection of Drosophila Embryos for RNA Interference (RNAi)

  • Leonie Misquitta,
  • Qin Wei,
  • and Bruce M. Paterson

  Cold Spring Harb Protoc; 2008; doi:10.1101/pdb.prot4917
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  Injection of dsRNA into Drosophila Embryos for RNA Interference (RNAi)

  • Leonie Misquitta,
  • Qin Wei,
  • and Bruce M. Paterson

  Cold Spring Harb Protoc; 2008; doi:10.1101/pdb.prot4918
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  Mounting Live Cells Attached to Coverslips for Microscopy

  • Andrew H. Fischer,
  • Kenneth A. Jacobson,
  • Jack Rose,
  • and Rolf Zeller

  Cold Spring Harb Protoc; 2008; doi:10.1101/pdb.prot4927
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  Labeling Acetylcholine Receptors in Live Cells Using Rhodamine α-Bungarotoxin for Imaging

  • Brad Chazotte

  Cold Spring Harb Protoc; 2008; doi:10.1101/pdb.prot4928
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  Labeling the Components of the Plasma Membrane with Fluorescent Dyes for Imaging

  • Brad Chazotte

  Cold Spring Harb Protoc; 2008; doi:10.1101/pdb.prot4930
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  Labeling the Golgi Apparatus with BODIPY-FL-Ceramide (C₅-DMB-ceramide) for Imaging

  • Brad Chazotte

  Cold Spring Harb Protoc; 2008; doi:10.1101/pdb.prot4931
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  Calculation of Spot Reliability Evaluation Scores (SRED) for DNA Microarray Data

  • Kazuro Shimokawa,
  • Rimantas Kodzius,
  • Yonehiro Matsumura,
  • and Yoshihide Hayashizaki

  Cold Spring Harb Protoc; 2008; doi:10.1101/pdb.prot4937
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  Calculation of Absolute Expression Values for DNA Microarray Data

  • Kazuro Shimokawa,
  • Rimantas Kodzius,
  • Yonehiro Matsumura,
  • and Yoshihide Hayashizaki

  Cold Spring Harb Protoc; 2008; doi:10.1101/pdb.prot4938
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  In Vitro Histone Methyltransferase Assay

  • Ian M. Fingerman,
  • Hai-Ning Du,
  • and Scott D. Briggs

  Cold Spring Harb Protoc; 2008; doi:10.1101/pdb.prot4939
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  Stable Isotope Labeling of Mammals (SILAM)

  • Daniel B. McClatchy and
  • John R. Yates III

  Cold Spring Harb Protoc; 2008; doi:10.1101/pdb.prot4940
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  Fixation, Embedding, and Sectioning of Plant Tissues

  • Detlef Weigel and
  • Jane Glazebrook

  Cold Spring Harb Protoc; 2008; doi:10.1101/pdb.prot4941
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  Preparation and Hydrolysis of Digoxygenin-Labeled Probes for In Situ Hybridization of Plant Tissues

  • Cristina Ferrandiz and
  • Allen Sessions

  Cold Spring Harb Protoc; 2008; doi:10.1101/pdb.prot4942
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  Nonradioactive In Situ Hybridization of RNA Probes to Sections of Plant Tissues

  • Cristina Ferrandiz and
  • Allen Sessions

  Cold Spring Harb Protoc; 2008; doi:10.1101/pdb.prot4943
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  Whole-Mount In Situ Hybridization of RNA Probes to Plant Tissues

  • Jan Traas

  Cold Spring Harb Protoc; 2008; doi:10.1101/pdb.prot4944
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  Immunohistochemistry on Sections of Plant Tissues Using Enzyme-Coupled Avidin-Biotin Complex

  • Detlef Weigel and
  • Jane Glazebrook

  Cold Spring Harb Protoc; 2008; doi:10.1101/pdb.prot4945
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  Immunohistochemistry on Sections of Plant Tissues Using Alkaline-Phosphatase-Coupled Secondary Antibody

  • Allen Sessions

  Cold Spring Harb Protoc; 2008; doi:10.1101/pdb.prot4946
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  Whole-Mount Fluorescence Immunocytochemistry on Xenopus Embryos

  • Chanjae Lee,
  • Esther Kieserman,
  • Ryan S. Gray,
  • Tae Joo Park,
  • and John Wallingford

  Cold Spring Harb Protoc; 2008; doi:10.1101/pdb.prot4957
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