Table of Contents

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Generation of High-Titer Lentivirus for the Production of Transgenic Quail

Greg Poynter,
David Huss,
and Rusty Lansford

Cold Spring Harb Protoc; 2009; doi:10.1101/pdb.prot5117

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Injection of Lentivirus into Stage-X Blastoderm for the Production of Transgenic Quail

Greg Poynter,
David Huss,
and Rusty Lansford

Cold Spring Harb Protoc; 2009; doi:10.1101/pdb.prot5118

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Screening for Transgenic Japanese Quail Offspring

Greg Poynter,
David Huss,
and Rusty Lansford

Cold Spring Harb Protoc; 2009; doi:10.1101/pdb.prot5119

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Assay for Human Rad51-Mediated DNA Displacement Loop Formation

Steven Raynard and
Patrick Sung

Cold Spring Harb Protoc; 2009; doi:10.1101/pdb.prot5120

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In Vitro Sumoylation of Recombinant Proteins and Subsequent Purification for Use in Enzymatic Assays

Vasupradha Vethantham and
James L. Manley

Cold Spring Harb Protoc; 2009; doi:10.1101/pdb.prot5121

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Culturing Lamprey Embryos

Natalya Nikitina,
Marianne Bronner-Fraser,
and Tatjana Sauka-Spengler

Cold Spring Harb Protoc; 2009; doi:10.1101/pdb.prot5122

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Microinjection of RNA and Morpholino Oligos into Lamprey Embryos

Natalya Nikitina,
Marianne Bronner-Fraser,
and Tatjana Sauka-Spengler

Cold Spring Harb Protoc; 2009; doi:10.1101/pdb.prot5123

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DiI Cell Labeling in Lamprey Embryos

Natalya Nikitina,
Marianne Bronner-Fraser,
and Tatjana Sauka-Spengler

Cold Spring Harb Protoc; 2009; doi:10.1101/pdb.prot5124

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Whole-Mount In Situ Hybridization on Lamprey Embryos

Natalya Nikitina,
Marianne Bronner-Fraser,
and Tatjana Sauka-Spengler

Cold Spring Harb Protoc; 2009; doi:10.1101/pdb.prot5125

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Immunostaining of Whole-Mount and Sectioned Lamprey Embryos

Natalya Nikitina,
Marianne Bronner-Fraser,
and Tatjana Sauka-Spengler

Cold Spring Harb Protoc; 2009; doi:10.1101/pdb.prot5126

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Fixation and Dissection of Parhyale hawaiensis Embryos

E. Jay Rehm,
Roberta L. Hannibal,
R. Crystal Chaw,
Mario A. Vargas-Vila,
and Nipam H. Patel

Cold Spring Harb Protoc; 2009; doi:10.1101/pdb.prot5127

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Injection of Parhyale hawaiensis Blastomeres with Fluorescently Labeled Tracers

E. Jay Rehm,
Roberta L. Hannibal,
R. Crystal Chaw,
Mario A. Vargas-Vila,
and Nipam H. Patel

Cold Spring Harb Protoc; 2009; doi:10.1101/pdb.prot5128

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Antibody Staining of Parhyale hawaiensis Embryos

E. Jay Rehm,
Roberta L. Hannibal,
R. Crystal Chaw,
Mario A. Vargas-Vila,
and Nipam H. Patel

Cold Spring Harb Protoc; 2009; doi:10.1101/pdb.prot5129

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In Situ Hybridization of Labeled RNA Probes to Fixed Parhyale hawaiensis Embryos

E. Jay Rehm,
Roberta L. Hannibal,
R. Crystal Chaw,
Mario A. Vargas-Vila,
and Nipam H. Patel

Cold Spring Harb Protoc; 2009; doi:10.1101/pdb.prot5130

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Preparation of dsRNA for Microinjection Experiments in Mouse

Petr Svoboda

Cold Spring Harb Protoc; 2009; doi:10.1101/pdb.prot5131

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Microinjection of dsRNA into Fully-Grown Mouse Oocytes

Paula Stein

Cold Spring Harb Protoc; 2009; doi:10.1101/pdb.prot5132

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Microinjection of dsRNA into Mouse One-Cell Embryos

Paula Stein

Cold Spring Harb Protoc; 2009; doi:10.1101/pdb.prot5133

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Cloning a Transgene for Transgenic RNAi in Mouse Oocytes

Petr Svoboda

Cold Spring Harb Protoc; 2009; doi:10.1101/pdb.prot5134

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Microinjection of Plasmids into Meiotically Incompetent Mouse Oocytes

Paula Stein

Cold Spring Harb Protoc; 2009; doi:10.1101/pdb.prot5135

Emerging Model Organisms

Japanese Quail: An Efficient Animal Model for the Production of Transgenic Avians

The Sea Lamprey Petromyzon marinus: A Model for Evolutionary and Developmental Biology

The Crustacean Parhyale hawaiensis: A New Model for Arthropod Development

Topic Introductions

RNAi Experiments in Mouse Oocytes and Early Embryos

Information Panels

Choosing the Sequence of dsRNA for RNAi in Mouse

Cloning and Sequencing an Inverted Repeat

Protocols

Generation of High-Titer Lentivirus for the Production of Transgenic Quail

Injection of Lentivirus into Stage-X Blastoderm for the Production of Transgenic Quail

Screening for Transgenic Japanese Quail Offspring

Assay for Human Rad51-Mediated DNA Displacement Loop Formation

In Vitro Sumoylation of Recombinant Proteins and Subsequent Purification for Use in Enzymatic Assays

Culturing Lamprey Embryos

Microinjection of RNA and Morpholino Oligos into Lamprey Embryos

DiI Cell Labeling in Lamprey Embryos

Whole-Mount In Situ Hybridization on Lamprey Embryos

Immunostaining of Whole-Mount and Sectioned Lamprey Embryos

Fixation and Dissection of Parhyale hawaiensis Embryos

Injection of Parhyale hawaiensis Blastomeres with Fluorescently Labeled Tracers

Antibody Staining of Parhyale hawaiensis Embryos

In Situ Hybridization of Labeled RNA Probes to Fixed Parhyale hawaiensis Embryos

Preparation of dsRNA for Microinjection Experiments in Mouse

Microinjection of dsRNA into Fully-Grown Mouse Oocytes

Microinjection of dsRNA into Mouse One-Cell Embryos

Cloning a Transgene for Transgenic RNAi in Mouse Oocytes

Microinjection of Plasmids into Meiotically Incompetent Mouse Oocytes

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