Table of Contents

February 2009; 2009 (2)

Emerging Model Organisms

  • The Moss Physcomitrella patens: A Novel Model System for Plant Development and Genomic Studies

    • David J. Cove,
    • Pierre-François Perroud,
    • Audra J. Charron,
    • Stuart F. McDaniel,
    • Abha Khandelwal,
    • and Ralph S. Quatrano
    Cold Spring Harb Protoc; 2009; doi:10.1101/pdb.emo115

  • The Choanoflagellates: Heterotrophic Nanoflagellates and Sister Group of the Metazoa

    • Nicole King,
    • Susan L. Young,
    • Monika Abedin,
    • Martin Carr,
    • and Barry S.C. Leadbeater
    Cold Spring Harb Protoc; 2009; doi:10.1101/pdb.emo116

Topic Introductions

  • Basics of Photoactivation

    • Graham C.R. Ellis-Davies
    Cold Spring Harb Protoc; 2009; doi:10.1101/pdb.top55

Protocols

  • In-Gel Trypsin Digest of Gel-Fractionated Proteins

    • Andrew J. Link and
    • Joshua LaBaer
    Cold Spring Harb Protoc; 2009; doi:10.1101/pdb.prot5110

  • Culturing the Moss Physcomitrella patens

    • David J. Cove,
    • Pierre-François Perroud,
    • Audra J. Charron,
    • Stuart F. McDaniel,
    • Abha Khandelwal,
    • and Ralph S. Quatrano
    Cold Spring Harb Protoc; 2009; doi:10.1101/pdb.prot5136

  • Chromosome Conformation Capture

    • Nathan F. Cope and
    • Peter Fraser
    Cold Spring Harb Protoc; 2009; doi:10.1101/pdb.prot5137

  • DNA Extraction from Formalin-Fixed, Paraffin-Embedded Tissue

    • Weining Tang,
    • Freedom B. David,
    • Malania M. Wilson,
    • Benjamin G. Barwick,
    • Brian R. Leyland-Jones,
    • and Mark M. Bouzyk
    Cold Spring Harb Protoc; 2009; doi:10.1101/pdb.prot5138

  • Virus-Induced Gene Silencing as a Tool for Delivery of dsRNA into Plants

    • Meenu Padmanabhan and
    • Savithramma P. Dinesh-Kumar
    Cold Spring Harb Protoc; 2009; doi:10.1101/pdb.prot5139

  • Isolation and Regeneration of Protoplasts of the Moss Physcomitrella patens

    • David J. Cove,
    • Pierre-François Perroud,
    • Audra J. Charron,
    • Stuart F. McDaniel,
    • Abha Khandelwal,
    • and Ralph S. Quatrano
    Cold Spring Harb Protoc; 2009; doi:10.1101/pdb.prot5140

  • Somatic Hybridization in the Moss Physcomitrella patens Using PEG-Induced Protoplast Fusion

    • David J. Cove,
    • Pierre-François Perroud,
    • Audra J. Charron,
    • Stuart F. McDaniel,
    • Abha Khandelwal,
    • and Ralph S. Quatrano
    Cold Spring Harb Protoc; 2009; doi:10.1101/pdb.prot5141

  • Chemical and UV Mutagenesis of Spores and Protonemal Tissue from the Moss Physcomitrella patens

    • David J. Cove,
    • Pierre-François Perroud,
    • Audra J. Charron,
    • Stuart F. McDaniel,
    • Abha Khandelwal,
    • and Ralph S. Quatrano
    Cold Spring Harb Protoc; 2009; doi:10.1101/pdb.prot5142

  • Transformation of the Moss Physcomitrella patens Using Direct DNA Uptake by Protoplasts

    • David J. Cove,
    • Pierre-François Perroud,
    • Audra J. Charron,
    • Stuart F. McDaniel,
    • Abha Khandelwal,
    • and Ralph S. Quatrano
    Cold Spring Harb Protoc; 2009; doi:10.1101/pdb.prot5143

  • Transformation of the Moss Physcomitrella patens Using T-DNA Mutagenesis

    • David J. Cove,
    • Pierre-François Perroud,
    • Audra J. Charron,
    • Stuart F. McDaniel,
    • Abha Khandelwal,
    • and Ralph S. Quatrano
    Cold Spring Harb Protoc; 2009; doi:10.1101/pdb.prot5144

  • Transformation of Moss Physcomitrella patens Gametophytes Using a Biolistic Projectile Delivery System

    • David J. Cove,
    • Pierre-François Perroud,
    • Audra J. Charron,
    • Stuart F. McDaniel,
    • Abha Khandelwal,
    • and Ralph S. Quatrano
    Cold Spring Harb Protoc; 2009; doi:10.1101/pdb.prot5145

  • Isolation of DNA, RNA, and Protein from the Moss Physcomitrella patens Gametophytes

    • David J. Cove,
    • Pierre-François Perroud,
    • Audra J. Charron,
    • Stuart F. McDaniel,
    • Abha Khandelwal,
    • and Ralph S. Quatrano
    Cold Spring Harb Protoc; 2009; doi:10.1101/pdb.prot5146

  • Isolation of Single Choanoflagellate Cells from Field Samples and Establishment of Clonal Cultures

    • Nicole King,
    • Susan L. Young,
    • Monika Abedin,
    • Martin Carr,
    • and Barry S.C. Leadbeater
    Cold Spring Harb Protoc; 2009; doi:10.1101/pdb.prot5147

  • Starting and Maintaining Monosiga brevicollis Cultures

    • Nicole King,
    • Susan L. Young,
    • Monika Abedin,
    • Martin Carr,
    • and Barry S.C. Leadbeater
    Cold Spring Harb Protoc; 2009; doi:10.1101/pdb.prot5148

  • Long-Term Frozen Storage of Choanoflagellate Cultures

    • Nicole King,
    • Susan L. Young,
    • Monika Abedin,
    • Martin Carr,
    • and Barry S.C. Leadbeater
    Cold Spring Harb Protoc; 2009; doi:10.1101/pdb.prot5149

  • Visualizing the Subcellular Localization of Actin, β-Tubulin, and DNA in Monosiga brevicollis

    • Nicole King,
    • Susan L. Young,
    • Monika Abedin,
    • Martin Carr,
    • and Barry S.C. Leadbeater
    Cold Spring Harb Protoc; 2009; doi:10.1101/pdb.prot5150

  • Preparation of Total RNA from Monosiga brevicollis and Other Choanoflagellates

    • Nicole King,
    • Susan L. Young,
    • Monika Abedin,
    • Martin Carr,
    • and Barry S.C. Leadbeater
    Cold Spring Harb Protoc; 2009; doi:10.1101/pdb.prot5151

  • Rapid Preparation of Genomic DNA from Monosiga brevicollis and Other Choanoflagellates

    • Nicole King,
    • Susan L. Young,
    • Monika Abedin,
    • Martin Carr,
    • and Barry S.C. Leadbeater
    Cold Spring Harb Protoc; 2009; doi:10.1101/pdb.prot5152

  • Preparation of High-Molecular-Weight Genomic DNA from Monosiga brevicollis and Other Choanoflagellates

    • Nicole King,
    • Susan L. Young,
    • Monika Abedin,
    • Martin Carr,
    • and Barry S.C. Leadbeater
    Cold Spring Harb Protoc; 2009; doi:10.1101/pdb.prot5153

  • Separation of Choanoflagellate and Bacterial Genomic DNA

    • Nicole King,
    • Susan L. Young,
    • Monika Abedin,
    • Martin Carr,
    • and Barry S.C. Leadbeater
    Cold Spring Harb Protoc; 2009; doi:10.1101/pdb.prot5154

  • A Simple Free-Radical Polymerization Method for the Fabrication of Microscale Acrylonitrile Fibers for Cellular Probing

    • Matthew R. Guido
    Cold Spring Harb Protoc; 2009; doi:10.1101/pdb.prot5155

  • The OP9-DL1 System: Generation of T-Lymphocytes from Embryonic or Hematopoietic Stem Cells In Vitro

    • Roxanne Holmes and
    • Juan Carlos Zúñiga-Pflücker
    Cold Spring Harb Protoc; 2009; doi:10.1101/pdb.prot5156
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This Issue

February 2009; 2009 (2)
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  1. Emerging Model Organisms
  2. Topic Introductions
  3. Protocols

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