Table of Contents

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Dot Blot Assay for Determining Fusion Protein Antiserum Quality

Ilaria Rebay and
Richard G. Fehon

Cold Spring Harb Protoc; 2011; doi:10.1101/pdb.prot4998

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Determining Fusion Protein Antiserum Quality by Staining of Transfected Drosophila S2 Cultured Cells

Ilaria Rebay and
Richard G. Fehon

Cold Spring Harb Protoc; 2011; doi:10.1101/pdb.prot4999

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Preparing Thick Sections of Frozen Adult Drosophila Retinas for Microscopy

Tanya Wolff

Cold Spring Harb Protoc; 2011; doi:10.1101/pdb.prot5544

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Preparation of Extracts from Yeast

Richard J. Simpson

Cold Spring Harb Protoc; 2011; doi:10.1101/pdb.prot5545

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Multicolor Brainbow Imaging in Zebrafish

Y. Albert Pan,
Jean Livet,
Joshua R. Sanes,
Jeff W. Lichtman,
and Alexander F. Schier

Cold Spring Harb Protoc; 2011; doi:10.1101/pdb.prot5546

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Labeling Tetracysteine-Tagged Proteins with Biarsenical Dyes for Live Cell Imaging

Guido M. Gaietta,
Thomas J. Deerinck,
and Mark H. Ellisman

Cold Spring Harb Protoc; 2011; doi:10.1101/pdb.prot5547

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Fluorescence Photoconversion of Biarsenical-Labeled Cells for Correlated Electron Microscopy (EM)

Guido M. Gaietta,
Thomas J. Deerinck,
and Mark H. Ellisman

Cold Spring Harb Protoc; 2011; doi:10.1101/pdb.prot5548

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Electroretinogram Recordings of Drosophila

Patrick Dolph,
Amit Nair,
and Padinjat Raghu

Cold Spring Harb Protoc; 2011; doi:10.1101/pdb.prot5549

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Preparation of Dissociated Ommatidia from Drosophila

Patrick Dolph,
Amit Nair,
and Padinjat Raghu

Cold Spring Harb Protoc; 2011; doi:10.1101/pdb.prot5550

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Whole-Cell Patch-Clamp Analysis of Adult Drosophila Photoreceptors

Patrick Dolph,
Amit Nair,
and Padinjat Raghu

Cold Spring Harb Protoc; 2011; doi:10.1101/pdb.prot5551

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Whole-Genome Amplification Using Φ29 DNA Polymerase

Noël P. Burtt

Cold Spring Harb Protoc; 2011; doi:10.1101/pdb.prot5552

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Synthesis of Cationic Polysaccharides and Use for In Vitro Transfection

Ira Yudovin-Farber,
Hagit Eliyahu,
and Abraham J. Domb

Cold Spring Harb Protoc; 2011; doi:10.1101/pdb.prot5553

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Mounting Live Cells onto Microscope Slides

Brad Chazotte

Cold Spring Harb Protoc; 2011; doi:10.1101/pdb.prot5554

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Labeling Membranes with Carbocyanine Dyes (DiIs) as Phospholipid Analogs

Brad Chazotte

Cold Spring Harb Protoc; 2011; doi:10.1101/pdb.prot5555

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Labeling Nuclear DNA Using DAPI

Brad Chazotte

Cold Spring Harb Protoc; 2011; doi:10.1101/pdb.prot5556

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Labeling Nuclear DNA with Hoechst 33342

Brad Chazotte

Cold Spring Harb Protoc; 2011; doi:10.1101/pdb.prot5557

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Organ Culture and Immunostaining of Mouse Embryonic Kidneys

Hila Barak and
Scott C. Boyle

Cold Spring Harb Protoc; 2011; doi:10.1101/pdb.prot5558

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NanoCAGE: A High-Resolution Technique to Discover and Interrogate Cell Transcriptomes

Md Salimullah,
Sakai Mizuho,
Charles Plessy,
and Piero Carninci

Cold Spring Harb Protoc; 2011; doi:10.1101/pdb.prot5559

Topic Introductions

Correlated Live Cell Light and Electron Microscopy Using Tetracysteine Tags and Biarsenicals

Multiple Testing and Power Calculations in Genetic Association Studies

Protocols

Dot Blot Assay for Determining Fusion Protein Antiserum Quality

Determining Fusion Protein Antiserum Quality by Staining of Transfected Drosophila S2 Cultured Cells

Preparing Thick Sections of Frozen Adult Drosophila Retinas for Microscopy

Preparation of Extracts from Yeast

Multicolor Brainbow Imaging in Zebrafish

Labeling Tetracysteine-Tagged Proteins with Biarsenical Dyes for Live Cell Imaging

Fluorescence Photoconversion of Biarsenical-Labeled Cells for Correlated Electron Microscopy (EM)

Electroretinogram Recordings of Drosophila

Preparation of Dissociated Ommatidia from Drosophila

Whole-Cell Patch-Clamp Analysis of Adult Drosophila Photoreceptors

Whole-Genome Amplification Using Φ29 DNA Polymerase

Synthesis of Cationic Polysaccharides and Use for In Vitro Transfection

Mounting Live Cells onto Microscope Slides

Labeling Membranes with Carbocyanine Dyes (DiIs) as Phospholipid Analogs

Labeling Nuclear DNA Using DAPI

Labeling Nuclear DNA with Hoechst 33342

Organ Culture and Immunostaining of Mouse Embryonic Kidneys

NanoCAGE: A High-Resolution Technique to Discover and Interrogate Cell Transcriptomes

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