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Preparation of Rat Serum for Culturing Mouse Embryos

Monica D. Garcia,
Ryan S. Udan,
Anna-Katerina Hadjantonakis,
and Mary E. Dickinson

Cold Spring Harb Protoc; 2011; doi:10.1101/pdb.prot5593

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Preparation of Postimplantation Mouse Embryos for Imaging

Monica D. Garcia,
Ryan S. Udan,
Anna-Katerina Hadjantonakis,
and Mary E. Dickinson

Cold Spring Harb Protoc; 2011; doi:10.1101/pdb.prot5594

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Time-Lapse Imaging of Postimplantation Mouse Embryos

Monica D. Garcia,
Ryan S. Udan,
Anna-Katerina Hadjantonakis,
and Mary E. Dickinson

Cold Spring Harb Protoc; 2011; doi:10.1101/pdb.prot5595

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Construction and Expression of Tetracysteine-Tagged Proteins for FlAsH-FALI

Ron L.P. Habets and
Patrik Verstreken

Cold Spring Harb Protoc; 2011; doi:10.1101/pdb.prot5596

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FlAsH-FALI Inactivation of a Protein at the Third-Instar Neuromuscular Junction

Ron L.P. Habets and
Patrik Verstreken

Cold Spring Harb Protoc; 2011; doi:10.1101/pdb.prot5597

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Production of SIV Vectors for Gene Delivery

Els Verhoeyen,
François-Loïc Cosset,
and Didier Nègre

Cold Spring Harb Protoc; 2011; doi:10.1101/pdb.prot5598

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Magnetic Twisting Cytometry

Karen E. Kasza,
David Vader,
Sarah Köster,
Ning Wang,
and David A. Weitz

Cold Spring Harb Protoc; 2011; doi:10.1101/pdb.prot5599

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Megaselia abdita: Culturing and Egg Collection

Ab. Matteen Rafiqi,
Steffen Lemke,
and Urs Schmidt-Ott

Cold Spring Harb Protoc; 2011; doi:10.1101/pdb.prot5600

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Megaselia abdita: Fixing and Devitellinizing Embryos

Ab. Matteen Rafiqi,
Steffen Lemke,
and Urs Schmidt-Ott

Cold Spring Harb Protoc; 2011; doi:10.1101/pdb.prot5602

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Megaselia abdita: Cuticle Preparation from Injected Embryos

Ab. Matteen Rafiqi,
Steffen Lemke,
and Urs Schmidt-Ott

Cold Spring Harb Protoc; 2011; doi:10.1101/pdb.prot5603

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Growing and Harvesting TAP-Tagged Yeast Cells

Andrew J. Link,
Connie Weaver,
and Adam Farley

Cold Spring Harb Protoc; 2011; doi:10.1101/pdb.prot5604

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Making Yeast Cell Extracts for Purifying TAP-tagged Protein Complexes

Andrew J. Link,
Connie Weaver,
and Adam Farley

Cold Spring Harb Protoc; 2011; doi:10.1101/pdb.prot5605

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IgG Affinity Capture of TAP-tagged Protein Complexes from Cell Extracts: Affinity Purification Step 1

Andrew J. Link,
Connie Weaver,
and Adam Farley

Cold Spring Harb Protoc; 2011; doi:10.1101/pdb.prot5606

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Affinity Capture of TAP-tagged Protein Complexes: Affinity Purification Step 2

Andrew J. Link,
Connie Weaver,
and Adam Farley

Cold Spring Harb Protoc; 2011; doi:10.1101/pdb.prot5607

Emerging Model Organisms

The Scuttle Fly Megaselia abdita (Phoridae): A Link between Drosophila and Mosquito Development

Topic Introductions

Imaging Gene Expression in Live Cells and Tissues

Live Imaging of Mouse Embryos

Maintaining Live Cells and Tissue Slices in the Imaging Setup

Imaging Techniques for Measuring the Materials Properties of Cells

Protocols

Preparation of Rat Serum for Culturing Mouse Embryos

Preparation of Postimplantation Mouse Embryos for Imaging

Time-Lapse Imaging of Postimplantation Mouse Embryos

Construction and Expression of Tetracysteine-Tagged Proteins for FlAsH-FALI

FlAsH-FALI Inactivation of a Protein at the Third-Instar Neuromuscular Junction

Production of SIV Vectors for Gene Delivery

Magnetic Twisting Cytometry

Megaselia abdita: Culturing and Egg Collection

Megaselia abdita: Fixing and Devitellinizing Embryos

Megaselia abdita: Cuticle Preparation from Injected Embryos

Growing and Harvesting TAP-Tagged Yeast Cells

Making Yeast Cell Extracts for Purifying TAP-tagged Protein Complexes

IgG Affinity Capture of TAP-tagged Protein Complexes from Cell Extracts: Affinity Purification Step 1

Affinity Capture of TAP-tagged Protein Complexes: Affinity Purification Step 2

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