Table of Contents

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Scoring and Analyzing Aggression in Drosophila

Sarah J. Certel and
Edward A. Kravitz

Cold Spring Harb Protoc; 2012; doi:10.1101/pdb.prot068130

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In Vivo Labeling of Cortical Astrocytes with Sulforhodamine 101 (SR101)

Axel Nimmerjahn and
Fritjof Helmchen

Cold Spring Harb Protoc; 2012; doi:10.1101/pdb.prot068155

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Long-Term Time-Lapse Imaging of Developing Hippocampal Neurons in Culture

Stefanie Kaech,
Chun-Fang Huang,
and Gary Banker

Cold Spring Harb Protoc; 2012; doi:10.1101/pdb.prot068239

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Short-Term High-Resolution Imaging of Developing Hippocampal Neurons in Culture

Stefanie Kaech,
Chun-Fang Huang,
and Gary Banker

Cold Spring Harb Protoc; 2012; doi:10.1101/pdb.prot068247

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Preparation of Chemotactically Competent Dictyostelium Cells

Satomi Matsuoka,
Yukihiro Miyanaga,
Toshio Yanagida,
and Masahiro Ueda

Cold Spring Harb Protoc; 2012; doi:10.1101/pdb.prot068197

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Preparation of an Imaging Chamber for Visualizing Single Molecules in Living Dictyostelium Cells

Satomi Matsuoka,
Yukihiro Miyanaga,
Toshio Yanagida,
and Masahiro Ueda

Cold Spring Harb Protoc; 2012; doi:10.1101/pdb.prot068205

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Visualizing Single Molecules in Living Dictyostelium Cells Using Total Internal Reflection Fluorescent Microscopy (TIRFM)

Satomi Matsuoka,
Yukihiro Miyanaga,
Toshio Yanagida,
and Masahiro Ueda

Cold Spring Harb Protoc; 2012; doi:10.1101/pdb.prot068213

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Construction and Packaging of Herpes simplex Virus/Adeno-Associated Virus (HSV/AAV) Hybrid Amplicon Vectors

Okay Saydam,
Daniel L. Glauser,
and Cornel Fraefel

Cold Spring Harb Protoc; 2012; doi:10.1101/pdb.prot068114

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Formation of Solid Lipid Nanoparticle (SLN)–Gene Vector Complexes for Transfection of Mammalian Cells In Vitro

Carsten Rudolph and
Joseph Rosenecker

Cold Spring Harb Protoc; 2012; doi:10.1101/pdb.prot068122

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Isolation and Regeneration of Protoplasts from Ectocarpus

Susana M. Coelho,
Delphine Scornet,
Sylvie Rousvoal,
Nick Peters,
Laurence Dartevelle,
Akira F. Peters,
and J. Mark Cock

Cold Spring Harb Protoc; 2012; doi:10.1101/pdb.prot067959

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Extraction of High-Quality Genomic DNA from Ectocarpus

Susana M. Coelho,
Delphine Scornet,
Sylvie Rousvoal,
Nick Peters,
Laurence Dartevelle,
Akira F. Peters,
and J. Mark Cock

Cold Spring Harb Protoc; 2012; doi:10.1101/pdb.prot067967

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Immunostaining of Ectocarpus Cells

Susana M. Coelho,
Delphine Scornet,
Sylvie Rousvoal,
Nick Peters,
Laurence Dartevelle,
Akira F. Peters,
and J. Mark Cock

Cold Spring Harb Protoc; 2012; doi:10.1101/pdb.prot067975

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Preparation and Mounting of Adult Drosophila Structures in Canada Balsam

David L. Stern and
Elio Sucena

Cold Spring Harb Protoc; 2012; doi:10.1101/pdb.prot067389

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Microdissection of Drosophila Polytene Chromosomes for DOP-PCR

Abby F. Dernburg

Cold Spring Harb Protoc; 2012; doi:10.1101/pdb.prot067298

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DOP-PCR Amplification of Probe DNA for Whole-Mount FISH in Drosophila

Abby F. Dernburg

Cold Spring Harb Protoc; 2012; doi:10.1101/pdb.prot067306

TOPIC INTRODUCTIONS

Single-Molecule Imaging of Stochastic Signaling Events in Living Cells

Identifying cis-Acting DNA Elements within a Control Region

Introduction to Genetic Association Studies

INFORMATION PANELS

Interferometric Detection of Action Potentials

General Considerations for Live Imaging of Developing Hippocampal Neurons in Culture

PROTOCOLS

Scoring and Analyzing Aggression in Drosophila

In Vivo Labeling of Cortical Astrocytes with Sulforhodamine 101 (SR101)

Long-Term Time-Lapse Imaging of Developing Hippocampal Neurons in Culture

Short-Term High-Resolution Imaging of Developing Hippocampal Neurons in Culture

Preparation of Chemotactically Competent Dictyostelium Cells

Preparation of an Imaging Chamber for Visualizing Single Molecules in Living Dictyostelium Cells

Visualizing Single Molecules in Living Dictyostelium Cells Using Total Internal Reflection Fluorescent Microscopy (TIRFM)

Construction and Packaging of Herpes simplex Virus/Adeno-Associated Virus (HSV/AAV) Hybrid Amplicon Vectors

Formation of Solid Lipid Nanoparticle (SLN)–Gene Vector Complexes for Transfection of Mammalian Cells In Vitro

Isolation and Regeneration of Protoplasts from Ectocarpus

Extraction of High-Quality Genomic DNA from Ectocarpus

Immunostaining of Ectocarpus Cells

Preparation and Mounting of Adult Drosophila Structures in Canada Balsam

Microdissection of Drosophila Polytene Chromosomes for DOP-PCR

DOP-PCR Amplification of Probe DNA for Whole-Mount FISH in Drosophila

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