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Retrograde Labeling of Corticospinal Motor Neurons from Early Postnatal Rodents

Wim Mandemakers

Cold Spring Harb Protoc; 2014; doi:10.1101/pdb.prot074922

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Immunopanning of Retrograde-Labeled Corticospinal Motor Neurons from Early Postnatal Rodents

Wim Mandemakers

Cold Spring Harb Protoc; 2014; doi:10.1101/pdb.prot074930

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Whole-Cell Patch-Clamp Recordings of Ca²⁺ Currents from Isolated Neonatal Mouse Dorsal Root Ganglion (DRG) Neurons

María A. Gandini,
Alejandro Sandoval,
and Ricardo Felix

Cold Spring Harb Protoc; 2014; doi:10.1101/pdb.prot073205

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Whole-Cell Patch-Clamp Recording of Recombinant Voltage-Sensitive Ca²⁺ Channels Heterologously Expressed in HEK-293 Cells

María A. Gandini,
Alejandro Sandoval,
and Ricardo Felix

Cold Spring Harb Protoc; 2014; doi:10.1101/pdb.prot073213

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In Vivo Two-Photon Calcium Imaging in the Visual System

Kenichi Ohki and
R. Clay Reid

Cold Spring Harb Protoc; 2014; doi:10.1101/pdb.prot081455

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Whole-Mount X-Gal Staining of Mouse Tissues

Jessica J. Gierut,
Tyler E. Jacks,
and Kevin M. Haigis

Cold Spring Harb Protoc; 2014; doi:10.1101/pdb.prot073452

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Fluorescent Visualization of Germline Apoptosis in Living Caenorhabditis elegans

Benjamin Lant and
W. Brent Derry

Cold Spring Harb Protoc; 2014; doi:10.1101/pdb.prot080226

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High-Throughput RNAi Screening for Germline Apoptosis Genes in Caenorhabditis elegans

Benjamin Lant and
W. Brent Derry

Cold Spring Harb Protoc; 2014; doi:10.1101/pdb.prot080234

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Electrophoretic Mobility Shift Assays for RNA–Protein Complexes

Donald C. Rio

Cold Spring Harb Protoc; 2014; doi:10.1101/pdb.prot080721

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5′-End Labeling of RNA with [γ-³²P]ATP and T4 Polynucleotide Kinase

Donald C. Rio

Cold Spring Harb Protoc; 2014; doi:10.1101/pdb.prot080739

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3′-End Labeling of RNA with [5′-³²P]Cytidine 3′,5′-Bis(Phosphate) and T4 RNA Ligase 1

Timothy W. Nilsen

Cold Spring Harb Protoc; 2014; doi:10.1101/pdb.prot080713

TOPIC INTRODUCTIONS

Patch-Clamp Recording of Voltage-Sensitive Ca²⁺ Channels

Purification and Culture of Corticospinal Motor Neurons

Strategies to Achieve Conditional Gene Mutation in Mice

Cross-Species Analysis of Mouse and Human Cancer Genomes

Video Microscopy, Video Cameras, and Image Enhancement

PROTOCOLS

Retrograde Labeling of Corticospinal Motor Neurons from Early Postnatal Rodents

Immunopanning of Retrograde-Labeled Corticospinal Motor Neurons from Early Postnatal Rodents

Whole-Cell Patch-Clamp Recordings of Ca²⁺ Currents from Isolated Neonatal Mouse Dorsal Root Ganglion (DRG) Neurons

Whole-Cell Patch-Clamp Recording of Recombinant Voltage-Sensitive Ca²⁺ Channels Heterologously Expressed in HEK-293 Cells

In Vivo Two-Photon Calcium Imaging in the Visual System

Whole-Mount X-Gal Staining of Mouse Tissues

Fluorescent Visualization of Germline Apoptosis in Living Caenorhabditis elegans

High-Throughput RNAi Screening for Germline Apoptosis Genes in Caenorhabditis elegans

Electrophoretic Mobility Shift Assays for RNA–Protein Complexes

5′-End Labeling of RNA with [γ-³²P]ATP and T4 Polynucleotide Kinase

3′-End Labeling of RNA with [5′-³²P]Cytidine 3′,5′-Bis(Phosphate) and T4 RNA Ligase 1

This Issue

Current Issue

From the cover

Table of Contents

TOPIC INTRODUCTIONS

Patch-Clamp Recording of Voltage-Sensitive Ca2+ Channels

Purification and Culture of Corticospinal Motor Neurons

Strategies to Achieve Conditional Gene Mutation in Mice

Cross-Species Analysis of Mouse and Human Cancer Genomes

Video Microscopy, Video Cameras, and Image Enhancement

PROTOCOLS

Retrograde Labeling of Corticospinal Motor Neurons from Early Postnatal Rodents

Immunopanning of Retrograde-Labeled Corticospinal Motor Neurons from Early Postnatal Rodents

Whole-Cell Patch-Clamp Recordings of Ca2+ Currents from Isolated Neonatal Mouse Dorsal Root Ganglion (DRG) Neurons

Whole-Cell Patch-Clamp Recording of Recombinant Voltage-Sensitive Ca2+ Channels Heterologously Expressed in HEK-293 Cells

In Vivo Two-Photon Calcium Imaging in the Visual System

Whole-Mount X-Gal Staining of Mouse Tissues

Fluorescent Visualization of Germline Apoptosis in Living Caenorhabditis elegans

High-Throughput RNAi Screening for Germline Apoptosis Genes in Caenorhabditis elegans

Electrophoretic Mobility Shift Assays for RNA–Protein Complexes

5′-End Labeling of RNA with [γ-32P]ATP and T4 Polynucleotide Kinase

3′-End Labeling of RNA with [5′-32P]Cytidine 3′,5′-Bis(Phosphate) and T4 RNA Ligase 1

This Issue

Current Issue

From the cover

Patch-Clamp Recording of Voltage-Sensitive Ca²⁺ Channels

Whole-Cell Patch-Clamp Recordings of Ca²⁺ Currents from Isolated Neonatal Mouse Dorsal Root Ganglion (DRG) Neurons

Whole-Cell Patch-Clamp Recording of Recombinant Voltage-Sensitive Ca²⁺ Channels Heterologously Expressed in HEK-293 Cells

5′-End Labeling of RNA with [γ-³²P]ATP and T4 Polynucleotide Kinase

3′-End Labeling of RNA with [5′-³²P]Cytidine 3′,5′-Bis(Phosphate) and T4 RNA Ligase 1