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Combinatorial Analysis of mRNA Expression Patterns in Mouse Embryos Using Hybridization Chain Reaction

David Huss,
Harry M.T. Choi,
Carol Readhead,
Scott E. Fraser,
Niles A. Pierce,
and Rusty Lansford

Cold Spring Harb Protoc; 2015; doi:10.1101/pdb.prot083832

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Tamoxifen Administration to Mice

Jonathan Whitfield,
Trevor Littlewood,
and Laura Soucek

Cold Spring Harb Protoc; 2015; doi:10.1101/pdb.prot077966

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Evaluating Yeast Filamentous Growth at the Single-Cell Level

Paul J. Cullen

Cold Spring Harb Protoc; 2015; doi:10.1101/pdb.prot085084

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Evaluating the Activity of the Filamentous Growth Mitogen-Activated Protein Kinase Pathway in Yeast

Paul J. Cullen

Cold Spring Harb Protoc; 2015; doi:10.1101/pdb.prot085092

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Electroporation Loading of Membrane-Impermeable Molecules to Investigate Intra- and Intercellular Ca²⁺ Signaling

Elke Decrock,
Marijke De Bock,
Nan Wang,
Mélissa Bol,
Ashish K. Gadicherla,
and Luc Leybaert

Cold Spring Harb Protoc; 2015; doi:10.1101/pdb.prot076562

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Flash Photolysis of Caged IP₃ to Trigger Intercellular Ca²⁺ Waves

Elke Decrock,
Marijke De Bock,
Nan Wang,
Mélissa Bol,
Ashish K. Gadicherla,
and Luc Leybaert

Cold Spring Harb Protoc; 2015; doi:10.1101/pdb.prot076570

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Isolation of Ribosomes and Polysomes

Maria C. Rivera,
Bruce Maguire,
and James A. Lake

Cold Spring Harb Protoc; 2015; doi:10.1101/pdb.prot081331

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Purification of 70S Ribosomes

Maria C. Rivera,
Bruce Maguire,
and James A. Lake

Cold Spring Harb Protoc; 2015; doi:10.1101/pdb.prot081356

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Purification of Polysomes

Maria C. Rivera,
Bruce Maguire,
and James A. Lake

Cold Spring Harb Protoc; 2015; doi:10.1101/pdb.prot081364

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Northern Blots: Capillary Transfer of RNA from Agarose Gels and Filter Hybridization Using Standard Stringency Conditions

Donald C. Rio

Cold Spring Harb Protoc; 2015; doi:10.1101/pdb.prot081018

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An Alternative Method for Processing Northern Blots After Capillary Transfer

Timothy W. Nilsen

Cold Spring Harb Protoc; 2015; doi:10.1101/pdb.prot081026

TOPIC INTRODUCTIONS

The Estrogen Receptor Fusion System in Mouse Models: A Reversible Switch

Investigating Filamentous Growth and Biofilm/Mat Formation in Budding Yeast

Electroporation Loading and Flash Photolysis to Investigate Intra- and Intercellular Ca²⁺ Signaling

Multiphoton Excitation of Fluorescent Probes

PROTOCOLS

Combinatorial Analysis of mRNA Expression Patterns in Mouse Embryos Using Hybridization Chain Reaction

Tamoxifen Administration to Mice

Evaluating Yeast Filamentous Growth at the Single-Cell Level

Evaluating the Activity of the Filamentous Growth Mitogen-Activated Protein Kinase Pathway in Yeast

Electroporation Loading of Membrane-Impermeable Molecules to Investigate Intra- and Intercellular Ca²⁺ Signaling

Flash Photolysis of Caged IP₃ to Trigger Intercellular Ca²⁺ Waves

Isolation of Ribosomes and Polysomes

Purification of 70S Ribosomes

Purification of Polysomes

Northern Blots: Capillary Transfer of RNA from Agarose Gels and Filter Hybridization Using Standard Stringency Conditions

An Alternative Method for Processing Northern Blots After Capillary Transfer

This Issue

Current Issue

From the cover

Table of Contents

TOPIC INTRODUCTIONS

The Estrogen Receptor Fusion System in Mouse Models: A Reversible Switch

Investigating Filamentous Growth and Biofilm/Mat Formation in Budding Yeast

Electroporation Loading and Flash Photolysis to Investigate Intra- and Intercellular Ca2+ Signaling

Multiphoton Excitation of Fluorescent Probes

PROTOCOLS

Combinatorial Analysis of mRNA Expression Patterns in Mouse Embryos Using Hybridization Chain Reaction

Tamoxifen Administration to Mice

Evaluating Yeast Filamentous Growth at the Single-Cell Level

Evaluating the Activity of the Filamentous Growth Mitogen-Activated Protein Kinase Pathway in Yeast

Electroporation Loading of Membrane-Impermeable Molecules to Investigate Intra- and Intercellular Ca2+ Signaling

Flash Photolysis of Caged IP3 to Trigger Intercellular Ca2+ Waves

Isolation of Ribosomes and Polysomes

Purification of 70S Ribosomes

Purification of Polysomes

Northern Blots: Capillary Transfer of RNA from Agarose Gels and Filter Hybridization Using Standard Stringency Conditions

An Alternative Method for Processing Northern Blots After Capillary Transfer

This Issue

Current Issue

From the cover

Electroporation Loading and Flash Photolysis to Investigate Intra- and Intercellular Ca²⁺ Signaling

Electroporation Loading of Membrane-Impermeable Molecules to Investigate Intra- and Intercellular Ca²⁺ Signaling

Flash Photolysis of Caged IP₃ to Trigger Intercellular Ca²⁺ Waves