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Identification of RNA Cargoes by Antibody-Positioned RNA Amplification

Kevin Y. Miyashiro and
James Eberwine

Cold Spring Harb Protoc; 2015; doi:10.1101/pdb.prot072447

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Mapping Protein–Protein Interactions Using Yeast Two-Hybrid Assays

Jitender Mehla,
J. Harry Caufield,
and Peter Uetz

Cold Spring Harb Protoc; 2015; doi:10.1101/pdb.prot086157

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Creation of Primary Cell Lines from Lineage-Labeled Mouse Models of Cancer

Andrew D. Rhim

Cold Spring Harb Protoc; 2015; doi:10.1101/pdb.prot078287

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Organotypic Culture of Untransformed and Tumorigenic Primary Mammary Epithelial Cells

Martin Jechlinger

Cold Spring Harb Protoc; 2015; doi:10.1101/pdb.prot078295

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Subcellular Fractionation of the Brain: Preparation of Synaptosomes and Synaptic Vesicles

Gareth J.O. Evans

Cold Spring Harb Protoc; 2015; doi:10.1101/pdb.prot083469

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Investigating Bax Subcellular Localization and Membrane Integration

Grant Dewson

Cold Spring Harb Protoc; 2015; doi:10.1101/pdb.prot086447

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Investigating Bak/Bax Activating Conformation Change by Immunoprecipitation

Grant Dewson

Cold Spring Harb Protoc; 2015; doi:10.1101/pdb.prot086454

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Detection of Bak/Bax Activating Conformation Change by Intracellular Flow Cytometry

Grant Dewson

Cold Spring Harb Protoc; 2015; doi:10.1101/pdb.prot086462

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Investigating the Oligomerization of Bak and Bax during Apoptosis by Cysteine Linkage

Grant Dewson

Cold Spring Harb Protoc; 2015; doi:10.1101/pdb.prot086470

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Blue Native PAGE and Antibody Gel Shift to Assess Bak and Bax Conformation Change and Oligomerization

Grant Dewson

Cold Spring Harb Protoc; 2015; doi:10.1101/pdb.prot086488

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Measuring Ca²⁺ Sparks in Cardiac Myocytes

Niall Macquaide,
Virginie Bito,
and Karin R. Sipido

Cold Spring Harb Protoc; 2015; doi:10.1101/pdb.prot076984

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Assessing Ca²⁺-Removal Pathways in Cardiac Myocytes

Virginie Bito,
Karin R. Sipido,
and Niall Macquaide

Cold Spring Harb Protoc; 2015; doi:10.1101/pdb.prot076992

TOPIC INTRODUCTIONS

The Synaptosome as a Model System for Studying Synaptic Physiology

The Yeast Two-Hybrid System: A Tool for Mapping Protein–Protein Interactions

A Systematic Approach for Assessing Ca²⁺ Handling in Cardiac Myocytes

PROTOCOLS

Identification of RNA Cargoes by Antibody-Positioned RNA Amplification

Mapping Protein–Protein Interactions Using Yeast Two-Hybrid Assays

Creation of Primary Cell Lines from Lineage-Labeled Mouse Models of Cancer

Organotypic Culture of Untransformed and Tumorigenic Primary Mammary Epithelial Cells

Subcellular Fractionation of the Brain: Preparation of Synaptosomes and Synaptic Vesicles

Investigating Bax Subcellular Localization and Membrane Integration

Investigating Bak/Bax Activating Conformation Change by Immunoprecipitation

Detection of Bak/Bax Activating Conformation Change by Intracellular Flow Cytometry

Investigating the Oligomerization of Bak and Bax during Apoptosis by Cysteine Linkage

Blue Native PAGE and Antibody Gel Shift to Assess Bak and Bax Conformation Change and Oligomerization

Measuring Ca²⁺ Sparks in Cardiac Myocytes

Assessing Ca²⁺-Removal Pathways in Cardiac Myocytes

This Issue

Current Issue

From the cover

Table of Contents

TOPIC INTRODUCTIONS

The Synaptosome as a Model System for Studying Synaptic Physiology

The Yeast Two-Hybrid System: A Tool for Mapping Protein–Protein Interactions

A Systematic Approach for Assessing Ca2+ Handling in Cardiac Myocytes

PROTOCOLS

Identification of RNA Cargoes by Antibody-Positioned RNA Amplification

Mapping Protein–Protein Interactions Using Yeast Two-Hybrid Assays

Creation of Primary Cell Lines from Lineage-Labeled Mouse Models of Cancer

Organotypic Culture of Untransformed and Tumorigenic Primary Mammary Epithelial Cells

Subcellular Fractionation of the Brain: Preparation of Synaptosomes and Synaptic Vesicles

Investigating Bax Subcellular Localization and Membrane Integration

Investigating Bak/Bax Activating Conformation Change by Immunoprecipitation

Detection of Bak/Bax Activating Conformation Change by Intracellular Flow Cytometry

Investigating the Oligomerization of Bak and Bax during Apoptosis by Cysteine Linkage

Blue Native PAGE and Antibody Gel Shift to Assess Bak and Bax Conformation Change and Oligomerization

Measuring Ca2+ Sparks in Cardiac Myocytes

Assessing Ca2+-Removal Pathways in Cardiac Myocytes

This Issue

Current Issue

From the cover

A Systematic Approach for Assessing Ca²⁺ Handling in Cardiac Myocytes

Measuring Ca²⁺ Sparks in Cardiac Myocytes

Assessing Ca²⁺-Removal Pathways in Cardiac Myocytes