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Isolating Epithelial and Epithelial-to-Mesenchymal Transition Populations from Primary Tumors by Fluorescence-Activated Cell Sorting

Nicole M. Aiello,
Andrew D. Rhim,
and Ben Z. Stanger

Cold Spring Harb Protoc; 2016; doi:10.1101/pdb.prot078352

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Orthotopic Injection of Pancreatic Cancer Cells

Nicole M. Aiello,
Andrew D. Rhim,
and Ben Z. Stanger

Cold Spring Harb Protoc; 2016; doi:10.1101/pdb.prot078360

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CRISPR Technology for Genome Activation and Repression in Mammalian Cells

Dan Du and
Lei S. Qi

Cold Spring Harb Protoc; 2016; doi:10.1101/pdb.prot090175

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Preparation of the Nuclear Matrix for Parallel Microscopy and Biochemical Analyses

Rosemary H.C. Wilson,
Emma L. Hesketh,
and Dawn Coverley

Cold Spring Harb Protoc; 2016; doi:10.1101/pdb.prot083758

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Generation and Validation of MYTH Baits: iMYTH and tMYTH Variants

Jamie Snider and
Igor Stagljar

Cold Spring Harb Protoc; 2016; doi:10.1101/pdb.prot087817

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MYTH Screening: iMYTH and tMYTH Variants

Jamie Snider and
Igor Stagljar

Cold Spring Harb Protoc; 2016; doi:10.1101/pdb.prot087825

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Detection of Autophagy in Caenorhabditis elegans Using GFP::LGG-1 as an Autophagy Marker

Nicholas J. Palmisano and
Alicia Meléndez

Cold Spring Harb Protoc; 2016; doi:10.1101/pdb.prot086496

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Detecting Autophagy in Caenorhabditis elegans Embryos Using Markers of P Granule Degradation

Nicholas J. Palmisano and
Alicia Meléndez

Cold Spring Harb Protoc; 2016; doi:10.1101/pdb.prot086504

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Inducing RNAi in Caenorhabditis elegans by Injection of dsRNA

Christopher M. Hammell and
Gregory J. Hannon

Cold Spring Harb Protoc; 2016; doi:10.1101/pdb.prot086306

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Use of the BioGRID Database for Analysis of Yeast Protein and Genetic Interactions

Rose Oughtred,
Andrew Chatr-aryamontri,
Bobby-Joe Breitkreutz,
Christie S. Chang,
Jennifer M. Rust,
Chandra L. Theesfeld,
Sven Heinicke,
Ashton Breitkreutz,
Daici Chen,
Jodi Hirschman,
Nadine Kolas,
Michael S. Livstone,
Julie Nixon,
Lara O’Donnell,
Lindsay Ramage,
Andrew Winter,
Teresa Reguly,
Adnane Sellam,
Chris Stark,
Lorrie Boucher,
Kara Dolinski,
and Mike Tyers

Cold Spring Harb Protoc; 2016; doi:10.1101/pdb.prot088880

TOPIC INTRODUCTIONS

An Introduction to CRISPR Technology for Genome Activation and Repression in Mammalian Cells

The Nuclear Matrix: Fractionation Techniques and Analysis

Membrane Yeast Two-Hybrid (MYTH) Mapping of Full-Length Membrane Protein Interactions

Voltage-Sensitive Dye Imaging of Neocortical Activity

BioGRID: A Resource for Studying Biological Interactions in Yeast

PROTOCOLS

Isolating Epithelial and Epithelial-to-Mesenchymal Transition Populations from Primary Tumors by Fluorescence-Activated Cell Sorting

Orthotopic Injection of Pancreatic Cancer Cells

CRISPR Technology for Genome Activation and Repression in Mammalian Cells

Preparation of the Nuclear Matrix for Parallel Microscopy and Biochemical Analyses

Generation and Validation of MYTH Baits: iMYTH and tMYTH Variants

MYTH Screening: iMYTH and tMYTH Variants

Detection of Autophagy in Caenorhabditis elegans Using GFP::LGG-1 as an Autophagy Marker

Detecting Autophagy in Caenorhabditis elegans Embryos Using Markers of P Granule Degradation

Inducing RNAi in Caenorhabditis elegans by Injection of dsRNA

Use of the BioGRID Database for Analysis of Yeast Protein and Genetic Interactions

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