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Adeno-Associated Virus–Mediated Delivery of CRISPR–Cas Systems for Genome Engineering in Mammalian Cells

Thomas Gaj and
David V. Schaffer

Cold Spring Harb Protoc; 2016; doi:10.1101/pdb.prot086868

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Quantitation of Apoptosis and Necrosis by Annexin V Binding, Propidium Iodide Uptake, and Flow Cytometry

Lisa C. Crowley,
Brooke J. Marfell,
Adrian P. Scott,
and Nigel J. Waterhouse

Cold Spring Harb Protoc; 2016; doi:10.1101/pdb.prot087288

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Detecting Cleaved Caspase-3 in Apoptotic Cells by Flow Cytometry

Lisa C. Crowley and
Nigel J. Waterhouse

Cold Spring Harb Protoc; 2016; doi:10.1101/pdb.prot087312

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The Dihydrofolate Reductase Protein-Fragment Complementation Assay: A Survival-Selection Assay for Large-Scale Analysis of Protein–Protein Interactions

Stephen W. Michnick,
Emmanuel D. Levy,
Christian R. Landry,
Jacqueline Kowarzyk,
and Vincent Messier

Cold Spring Harb Protoc; 2016; doi:10.1101/pdb.prot090027

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Combining the Dihydrofolate Reductase Protein-Fragment Complementation Assay with Gene Deletions to Establish Genotype-to-Phenotype Maps of Protein Complexes and Interaction Networks

Guillaume Diss and
Christian R. Landry

Cold Spring Harb Protoc; 2016; doi:10.1101/pdb.prot090035

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Dissecting the Contingent Interactions of Protein Complexes with the Optimized Yeast Cytosine Deaminase Protein-Fragment Complementation Assay

Po Hien Ear,
Jacqueline Kowarzyk,
and Stephen W. Michnick

Cold Spring Harb Protoc; 2016; doi:10.1101/pdb.prot090043

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Real-Time Protein-Fragment Complementation Assays for Studying Temporal, Spatial, and Spatiotemporal Dynamics of Protein–Protein Interactions in Living Cells

Mohan Malleshaiah,
Emmanuelle Tchekanda,
and Stephen W. Michnick

Cold Spring Harb Protoc; 2016; doi:10.1101/pdb.prot090068

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Micrococcal Nuclease Digestion of Schizosaccharomyces pombe Chromatin

Hugh P. Cam and
Simon Whitehall

Cold Spring Harb Protoc; 2016; doi:10.1101/pdb.prot091538

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Chromatin Immunoprecipitation (ChIP) in Schizosaccharomyces pombe

Hugh P. Cam and
Simon Whitehall

Cold Spring Harb Protoc; 2016; doi:10.1101/pdb.prot091546

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Visualizing Genomic Annotations with the UCSC Genome Browser

Jui-Hung Hung and
Zhiping Weng

Cold Spring Harb Protoc; 2016; doi:10.1101/pdb.prot093062

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Sequence Alignment and Homology Search with BLAST and ClustalW

Jui-Hung Hung and
Zhiping Weng

Cold Spring Harb Protoc; 2016; doi:10.1101/pdb.prot093088

TOPIC INTRODUCTIONS

Protein-Fragment Complementation Assays for Large-Scale Analysis, Functional Dissection, and Spatiotemporal Dynamic Studies of Protein–Protein Interactions in Living Cells

Analysis of Heterochromatin in Schizosaccharomyces pombe

Transcription Factor–DNA Binding Motifs in Saccharomyces cerevisiae: Tools and Resources

Sequence Alignment and Homology Search

PROTOCOLS

Adeno-Associated Virus–Mediated Delivery of CRISPR–Cas Systems for Genome Engineering in Mammalian Cells

Quantitation of Apoptosis and Necrosis by Annexin V Binding, Propidium Iodide Uptake, and Flow Cytometry

Detecting Cleaved Caspase-3 in Apoptotic Cells by Flow Cytometry

The Dihydrofolate Reductase Protein-Fragment Complementation Assay: A Survival-Selection Assay for Large-Scale Analysis of Protein–Protein Interactions

Combining the Dihydrofolate Reductase Protein-Fragment Complementation Assay with Gene Deletions to Establish Genotype-to-Phenotype Maps of Protein Complexes and Interaction Networks

Dissecting the Contingent Interactions of Protein Complexes with the Optimized Yeast Cytosine Deaminase Protein-Fragment Complementation Assay

Real-Time Protein-Fragment Complementation Assays for Studying Temporal, Spatial, and Spatiotemporal Dynamics of Protein–Protein Interactions in Living Cells

Micrococcal Nuclease Digestion of Schizosaccharomyces pombe Chromatin

Chromatin Immunoprecipitation (ChIP) in Schizosaccharomyces pombe

Visualizing Genomic Annotations with the UCSC Genome Browser

Sequence Alignment and Homology Search with BLAST and ClustalW

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