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PROTOCOLS

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  Dissecting and Culturing Animal Cap Explants

  • Kevin S. Dingwell and
  • James C. Smith

  Cold Spring Harb Protoc; 2018; doi:10.1101/pdb.prot097329
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  Bulk Electroporation-Mediated Gene Transfer into Xenopus Tadpole Brain

  • Cristina Sáenz de Miera,
  • Ethan Parr,
  • and Robert J. Denver

  Cold Spring Harb Protoc; 2018; doi:10.1101/pdb.prot097691
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  DAPI Staining of Whole-Mount Mouse Embryos or Fetal Organs

  • Lisa Sandell,
  • Kimberly Inman,
  • and Paul Trainor

  Cold Spring Harb Protoc; 2018; doi:10.1101/pdb.prot094029
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  Immunofluorescent Staining of Whole-Mount Mouse Embryos

  • Allison Stewart

  Cold Spring Harb Protoc; 2018; doi:10.1101/pdb.prot094037
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  Whole-Mount Immunofluorescence for Visualizing Endogenous Protein and Injected RNA in Xenopus Oocytes

  • Samantha P. Jeschonek and
  • Kimberly L. Mowry

  Cold Spring Harb Protoc; 2018; doi:10.1101/pdb.prot097022
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  Oocyte Host-Transfer and Maternal mRNA Depletion Experiments in Xenopus

  • Douglas W. Houston

  Cold Spring Harb Protoc; 2018; doi:10.1101/pdb.prot096982
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  Making Weak Antigens Strong: Cross-Linking Peptides to KLH with Maleimide

  • Edward A. Greenfield,
  • James DeCaprio,
  • and Mohan Brahmandam

  Cold Spring Harb Protoc; 2018; doi:10.1101/pdb.prot100016
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  Resolving Proteins for Immunoblotting by Gel Electrophoresis

  • Larisa Litovchick

  Cold Spring Harb Protoc; 2018; doi:10.1101/pdb.prot098434
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  Immunoblotting: Transfer of Proteins from Gels to Membranes

  • Larisa Litovchick

  Cold Spring Harb Protoc; 2018; doi:10.1101/pdb.prot098442
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  Optimizing Primer and Probe Concentrations for Use in Real-Time Polymerase Chain Reaction (PCR) Assays

  • Michael R. Green and
  • Joseph Sambrook

  Cold Spring Harb Protoc; 2018; doi:10.1101/pdb.prot095018
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  Constructing a Standard Curve for Real-Time Polymerase Chain Reaction (PCR) Experiments

  • Michael R. Green and
  • Joseph Sambrook

  Cold Spring Harb Protoc; 2018; doi:10.1101/pdb.prot095026
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  Quantification of DNA by Real-Time Polymerase Chain Reaction (PCR)

  • Michael R. Green and
  • Joseph Sambrook

  Cold Spring Harb Protoc; 2018; doi:10.1101/pdb.prot095034
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  Quantification of RNA by Real-Time Reverse Transcription-Polymerase Chain Reaction (RT-PCR)

  • Michael R. Green and
  • Joseph Sambrook

  Cold Spring Harb Protoc; 2018; doi:10.1101/pdb.prot095042
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