Table of Contents

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Spemann–Mangold Grafts

Hélène Cousin

Cold Spring Harb Protoc; 2019; doi:10.1101/pdb.prot097345

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Einsteck Transplants

Hélène Cousin

Cold Spring Harb Protoc; 2019; doi:10.1101/pdb.prot097352

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Filopodia-Like Structure Formation from Xenopus Egg Extracts

Helen M. Fox and
Jennifer L. Gallop

Cold Spring Harb Protoc; 2019; doi:10.1101/pdb.prot100545

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Endoplasmic Reticulum Network Formation with Xenopus Egg Extracts

Songyu Wang,
Fabian B. Romano,
and Tom A. Rapoport

Cold Spring Harb Protoc; 2019; doi:10.1101/pdb.prot097204

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Chromosome Cohesion and Condensation in Xenopus Egg Extracts

Eulália M.L. da Silva and
Susannah Rankin

Cold Spring Harb Protoc; 2019; doi:10.1101/pdb.prot097121

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Chromatin Characterization in Xenopus laevis Cell-Free Egg Extracts and Embryos

Wei-Lin Wang,
Takashi Onikubo,
and David Shechter

Cold Spring Harb Protoc; 2019; doi:10.1101/pdb.prot099879

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Isolation of DNA Fragments from Polyacrylamide Gels by the Crush and Soak Method

Michael R. Green and
Joseph Sambrook

Cold Spring Harb Protoc; 2019; doi:10.1101/pdb.prot100479

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Tandem Immunoaffinity Purification Using Anti-FLAG and Anti-HA Antibodies

James DeCaprio and
Thomas O. Kohl

Cold Spring Harb Protoc; 2019; doi:10.1101/pdb.prot098657

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Cross-Linking Antibodies to Beads Using Dimethyl Pimelimidate (DMP)

James DeCaprio and
Thomas O. Kohl

Cold Spring Harb Protoc; 2019; doi:10.1101/pdb.prot098624

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Cross-Linking Antibodies to Beads with Disuccinimidyl Suberate (DSS)

James DeCaprio and
Thomas O. Kohl

Cold Spring Harb Protoc; 2019; doi:10.1101/pdb.prot098632

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Preparation of Mouse Tissue Lysates for Polymerase Chain Reaction

William Perry

Cold Spring Harb Protoc; 2019; doi:10.1101/pdb.prot094383

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Polymerase Chain Reaction (PCR) Amplification of GC-Rich Templates

Michael R. Green and
Joseph Sambrook

Cold Spring Harb Protoc; 2019; doi:10.1101/pdb.prot095141

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Inverse Polymerase Chain Reaction (PCR)

Michael R. Green and
Joseph Sambrook

Cold Spring Harb Protoc; 2019; doi:10.1101/pdb.prot095166

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Nested Polymerase Chain Reaction (PCR)

Michael R. Green and
Joseph Sambrook

Cold Spring Harb Protoc; 2019; doi:10.1101/pdb.prot095182

TOPIC INTRODUCTIONS

How to Win the Battle with RNase

PROTOCOLS

Spemann–Mangold Grafts

Einsteck Transplants

Filopodia-Like Structure Formation from Xenopus Egg Extracts

Endoplasmic Reticulum Network Formation with Xenopus Egg Extracts

Chromosome Cohesion and Condensation in Xenopus Egg Extracts

Chromatin Characterization in Xenopus laevis Cell-Free Egg Extracts and Embryos

Isolation of DNA Fragments from Polyacrylamide Gels by the Crush and Soak Method

Tandem Immunoaffinity Purification Using Anti-FLAG and Anti-HA Antibodies

Cross-Linking Antibodies to Beads Using Dimethyl Pimelimidate (DMP)

Cross-Linking Antibodies to Beads with Disuccinimidyl Suberate (DSS)

Preparation of Mouse Tissue Lysates for Polymerase Chain Reaction

Polymerase Chain Reaction (PCR) Amplification of GC-Rich Templates

Inverse Polymerase Chain Reaction (PCR)

Nested Polymerase Chain Reaction (PCR)

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