Table of Contents

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Long and Accurate Polymerase Chain Reaction (LA PCR)

Michael R. Green and
Joseph Sambrook

Cold Spring Harb Protoc; 2019; doi:10.1101/pdb.prot095158

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Preparation of Polymerase Chain Reaction Templates from Embryonic and Adult Mouse Tissue Samples

Janet Rossant and
Andras Nagy

Cold Spring Harb Protoc; 2019; doi:10.1101/pdb.prot094391

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Extracts for Analysis of DNA Replication in a Nucleus-Free System

Justin Sparks and
Johannes C. Walter

Cold Spring Harb Protoc; 2019; doi:10.1101/pdb.prot097154

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RNAi in Drosophila S2 Cells by dsRNA Soaking

Chengjian Li and
Phillip D. Zamore

Cold Spring Harb Protoc; 2019; doi:10.1101/pdb.prot097477

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Preparation of dsRNAs for RNAi by In Vitro Transcription

Chengjian Li and
Phillip D. Zamore

Cold Spring Harb Protoc; 2019; doi:10.1101/pdb.prot097469

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DNA Transfection Mediated by Cationic Lipid Reagents

Priti Kumar,
Arvindhan Nagarajan,
and Pradeep D. Uchil

Cold Spring Harb Protoc; 2019; doi:10.1101/pdb.prot095414

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Transfected Dendritic Cell Immunizations

Edward A. Greenfield,
James DeCaprio,
and Mohan Brahmandam

Cold Spring Harb Protoc; 2019; doi:10.1101/pdb.prot100073

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Histochemical Staining of Cell Monolayers for β-Galactosidase

Priti Kumar,
Arvindhan Nagarajan,
and Pradeep D. Uchil

Cold Spring Harb Protoc; 2019; doi:10.1101/pdb.prot095422

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Labeling Antibodies Using N-Hydroxysuccinimide (NHS)-Fluorescein

Eric A. Berg and
Jordan B. Fishman

Cold Spring Harb Protoc; 2019; doi:10.1101/pdb.prot099283

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Labeling Antibodies Using a Maleimido Dye

Eric A. Berg and
Jordan B. Fishman

Cold Spring Harb Protoc; 2019; doi:10.1101/pdb.prot099291

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Random Priming: Labeling of Purified DNA Fragments by Extension of Random Oligonucleotides

Michael R. Green and
Joseph Sambrook

Cold Spring Harb Protoc; 2019; doi:10.1101/pdb.prot100586

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Measurement of Radioactivity in Nucleic Acids

Michael R. Green and
Joseph Sambrook

Cold Spring Harb Protoc; 2019; doi:10.1101/pdb.prot100933

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Spun-Column Chromatography

Michael R. Green and
Joseph Sambrook

Cold Spring Harb Protoc; 2019; doi:10.1101/pdb.prot100594

TOPIC INTRODUCTIONS

Reconstituting Nuclear and Chromosome Dynamics Using Xenopus Extracts

Lipofection

PROTOCOLS

Long and Accurate Polymerase Chain Reaction (LA PCR)

Preparation of Polymerase Chain Reaction Templates from Embryonic and Adult Mouse Tissue Samples

Extracts for Analysis of DNA Replication in a Nucleus-Free System

RNAi in Drosophila S2 Cells by dsRNA Soaking

Preparation of dsRNAs for RNAi by In Vitro Transcription

DNA Transfection Mediated by Cationic Lipid Reagents

Transfected Dendritic Cell Immunizations

Histochemical Staining of Cell Monolayers for β-Galactosidase

Labeling Antibodies Using N-Hydroxysuccinimide (NHS)-Fluorescein

Labeling Antibodies Using a Maleimido Dye

Random Priming: Labeling of Purified DNA Fragments by Extension of Random Oligonucleotides

Measurement of Radioactivity in Nucleic Acids

Spun-Column Chromatography

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