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Following Endocrine-Disrupting Effects on Gene Expression in Xenopus laevis

Petra Spirhanzlova,
Michelle Leemans,
Barbara A. Demeneix,
and Jean-Baptiste Fini

Cold Spring Harb Protoc; 2019; doi:10.1101/pdb.prot098301

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Analysis of Phosphorylation Status of Ectopically Expressed Proteins in Early Xenopus Embryos

Laura J.A. Hardwick and
Anna Philpott

Cold Spring Harb Protoc; 2019; doi:10.1101/pdb.prot105569

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Analysis of Chromatin Binding of Ectopically Expressed Proteins in Early Xenopus Embryos

Laura J.A. Hardwick and
Anna Philpott

Cold Spring Harb Protoc; 2019; doi:10.1101/pdb.prot105577

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Freezing Cell Pellets for Large-Scale Immunoprecipitation

Larisa Litovchick

Cold Spring Harb Protoc; 2019; doi:10.1101/pdb.prot098541

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Using Dounce Homogenization to Lyse Cells for Immunoprecipitation

James DeCaprio and
Thomas O. Kohl

Cold Spring Harb Protoc; 2019; doi:10.1101/pdb.prot098574

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Detecting Gold-Labeled Cells

Scott J. Rodig

Cold Spring Harb Protoc; 2019; doi:10.1101/pdb.prot099754

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Detecting Iodine-Labeled Cells

Scott J. Rodig

Cold Spring Harb Protoc; 2019; doi:10.1101/pdb.prot099762

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DNA Transfection by Electroporation

Priti Kumar,
Arvindhan Nagarajan,
and Pradeep D. Uchil

Cold Spring Harb Protoc; 2019; doi:10.1101/pdb.prot095471

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Amplification of RNA for Microarrays

Oliver Rando

Cold Spring Harb Protoc; 2019; doi:10.1101/pdb.prot096420

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Direct Cyanine-dUTP Labeling of RNA for Microarrays

Oliver Rando

Cold Spring Harb Protoc; 2019; doi:10.1101/pdb.prot096438

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Indirect Aminoallyl-dUTP Labeling of RNA for Microarrays

Oliver Rando

Cold Spring Harb Protoc; 2019; doi:10.1101/pdb.prot096446

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Synthesis of cDNA Probes from mRNA Using Random Oligonucleotide Primers

Michael R. Green and
Joseph Sambrook

Cold Spring Harb Protoc; 2019; doi:10.1101/pdb.prot100636

TOPIC INTRODUCTION

Electroporation

PROTOCOLS

Following Endocrine-Disrupting Effects on Gene Expression in Xenopus laevis

Analysis of Phosphorylation Status of Ectopically Expressed Proteins in Early Xenopus Embryos

Analysis of Chromatin Binding of Ectopically Expressed Proteins in Early Xenopus Embryos

Freezing Cell Pellets for Large-Scale Immunoprecipitation

Using Dounce Homogenization to Lyse Cells for Immunoprecipitation

Detecting Gold-Labeled Cells

Detecting Iodine-Labeled Cells

DNA Transfection by Electroporation

Amplification of RNA for Microarrays

Direct Cyanine-dUTP Labeling of RNA for Microarrays

Indirect Aminoallyl-dUTP Labeling of RNA for Microarrays

Synthesis of cDNA Probes from mRNA Using Random Oligonucleotide Primers

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