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PROTOCOLS

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  Obtaining Polytene, Meiotic, and Mitotic Chromosomes from Mosquitoes for Cytogenetic Analysis

  • Jiangtao Liang,
  • Simon M. Bondarenko,
  • Igor V. Sharakhov,
  • and Maria V. Sharakhova

  Cold Spring Harb Protoc; 2022; doi:10.1101/pdb.prot107872
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  Visualization of Polytene Chromatin in Mosquito Cell Nuclei Using Three-Dimensional Fluorescence In Situ Hybridization

  • Simon M. Bondarenko,
  • Jiangtao Liang,
  • Maria V. Sharakhova,
  • and Igor V. Sharakhov

  Cold Spring Harb Protoc; 2022; doi:10.1101/pdb.prot107873
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  Homology-Directed Repair by CRISPR–Cas9 Mutagenesis in Xenopus Using Long Single-Stranded Donor DNA Templates via Simple Microinjection of Embryos

  • Takuya Nakayama,
  • Robert M. Grainger,
  • and Sang-Wook Cha

  Cold Spring Harb Protoc; 2022; doi:10.1101/pdb.prot107599
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  Gynogenetic Production of Embryos in Xenopus tropicalis Using a Cold Shock Procedure: Rapid Screening Method for Gene Editing Phenotypes

  • Takuya Nakayama,
  • Amanda Cox,
  • Mary Howell,
  • and Robert M. Grainger

  Cold Spring Harb Protoc; 2022; doi:10.1101/pdb.prot107648
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  Long-Term Brain Organotypic Cultures of the Turquoise Killifish Nothobranchius furzeri

  • Sara Bagnoli,
  • Letizia Brogi,
  • Baldassare Fronte,
  • Carlo Bibbiani,
  • Eva Terzibasi Tozzini,
  • and Alessandro Cellerino

  Cold Spring Harb Protoc; 2022; doi:10.1101/pdb.prot107746
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  Molecular Sexing of Nothobranchius furzeri Embryos and Larvae

  • Annekatrin Richter,
  • Johannes Krug,
  • and Christoph Englert

  Cold Spring Harb Protoc; 2022; doi:10.1101/pdb.prot107782
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  Preparation of Intact Nuclei for Single-Nucleus Omics Using Frozen Cell Suspensions from Mutant Embryos of Xenopus tropicalis

  • Takuya Nakayama,
  • Janou A.Y. Roubroeks,
  • Gert Jan C. Veenstra,
  • and Robert M. Grainger

  Cold Spring Harb Protoc; 2022; doi:10.1101/pdb.prot107825
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