Recipe

Supplemented Buffer P

 
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50 mM sodium phosphate (pH 7.4)

0.5 M NaCl

0.5 mg/ml lysozyme

1 mM phenylmethylsulfonyl fluoride (PMSF)

1.7 units/ml Benzonase (Merck, Darmstadt, Germany)

1 mM MgCl2

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Add the lysozyme, PMSF, and Benzonase just prior to use in Step 3.

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  1. doi:10.1101/pdb.rec10009 Cold Spring Harb Protoc 2006: pdb.rec10009-
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