Native separation medium 2

Glycerol (25% w/w)

Hydroxypropylmethyl cellulose (HPMC) (0.2% w/w)

ProLyte 2 (20% w/w)

For 210 g, weigh out 42 g of ProLyte 2. Add 10.5 g of H₂O and 157.5 g of HPMC/glycerol/H₂O stock (33%/33%/33% w/w/w). Mix thoroughly by stirring. pH = 6.98 ±0.10; conductivity 495 ±15 μS/cm. Actual Consumption: ˜27 g/hr.