Recipe

Native counterflow medium

 
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Glycerol (25% w/w)

Hydroxypropylmethyl cellulose (HPMC) (0.2% w/w)

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For 330 g, weigh out 247.5 g of HPMC/glycerol/H2O stock (33%/33%/33% w/w/w). Add 82.5 g of H2O. Mix thoroughly by stirring. Actual Consumption: ˜41 g/hr.

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  1. doi:10.1101/pdb.rec10123 Cold Spring Harb Protoc 2006: pdb.rec10123-
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