Recipe

Dissection medium

 
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DMEM/F12 (GIBCO 11330032), 45 ml

Heat-inactivated fetal bovine serum (FBS) (GIBCO), 4.5 ml

100x Pen-Strep solution (Sigma P 0781), 0.5 ml

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Sterilize using a 0.2-μm filter. Store at 4°C.

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  1. doi:10.1101/pdb.rec10364 Cold Spring Harb Protoc 2006: pdb.rec10364-
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