Recipe

Static embryo culture medium

 
Next Section

DMEM/F12 (GIBCO), 1 ml

Heat-inactivated rat serum, 1 ml

100x Pen-Strep (Sigma), 10 μl

HEPES buffer solution (1 M, pH 7.4; Irvine Scientific), 10 μl

Previous Section
 

Prepare rat serum as described in Preparation of Rat Serum except that rather than using a syringe to collect the blood, use a Vacutainer Safety-lok butterfly needle (Becton-Dickinson) and Vacutainer blood collection tubes (Becton-Dickinson).

| Table of Contents

This Article

  1. doi:10.1101/pdb.rec10365 Cold Spring Harb Protoc 2006: pdb.rec10365-
  1. » Full Text

Article Category

  1. Recipe

Personal Folder

  1. Save to Personal Folders

Updates/Comments

  1. Submit Updates/Comments
  2. No Updates/Comments published
  3. Alert me when Updates/Comments are published

Share

Navigate This Article

  1. Top

Current Issue

  1. March 2026, 2026 (3)
  1. Current Issue