Recipe

Denaturing counterflow media

 
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Glycerol (14.5% w/w)

Hydroxypropylmethyl cellulose (HPMC) (0.12% w/w)

8 M urea

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For 330.5 g = ~228 ml: Weigh out 120 g of HPMC/glycerol/H2O stock (40%/40%/20% w/w/w). Add 72 g of H2O and 138.5 g of urea. Dissolve by stirring in lukewarm water. Actual Consumption: ~46 g/hr.

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  1. doi:10.1101/pdb.rec10390 Cold Spring Harb Protoc 2006: pdb.rec10390-
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