Denaturing separation media 2

Glycerol (14.5% w/w)

Hydroxypropylmethyl cellulose (HPMC) (0.12% w/w)

8 M urea

ProLyte 2 (19.4% w/w)

For 137.8 g = ~120 ml: Weigh out 26.7 g of ProLyte 2. Add 3.3 g of H₂O and 50 g of HPMC/glycerol/H₂O stock (40%/40%/20% w/w/w). Add 57.8 g of urea. Dissolve by stirring in lukewarm water. pH = 7.54 ±0.10; conductivity 347 ±20 μS/cm. Actual Consumption: ˜20 g/hr.