Denaturing separation media 3

Glycerol (14.5% w/w)

Hydroxypropylmethyl cellulose (HPMC) (0.12% w/w)

8 M urea

ProLyte 3 (14.5% w/w)

For 68.9 g = ~60 ml: Weigh out 10 g of ProLyte 3. Add 5 g of H₂O and 25 g of HPMC/glycerol/H₂O stock (40%/40%/20% w/w/w). Add 28.9 g of urea. Dissolve by stirring in lukewarm water. pH = 10.26 ±0.17; conductivity 235 ±20 μS/cm. Actual Consumption: ˜10 g/hr.