Recipe

ES Freezing medium

 
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ES-DMEM

FBS

DMSO

Purchase a high grade of DMSO (HPLC grade or better).

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For a 2x solution:

60% ES-DMEM (containing 15% fetal bovine serum [FBS])

20% FBS

20% dimethyl sulfoxide (DMSO)

Prepare fresh; cool on ice.

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  1. doi:10.1101/pdb.rec10407 Cold Spring Harb Protoc 2006: pdb.rec10407-
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