Recipe

PCR buffer (Gittschier)

 
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16.6 mM (NH4)2SO4

67 mM Tris-Cl (pH 8.8)

6.7 mM MgCl2

5 mM β-mercaptoethanol

6.7 μM EDTA

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  1. doi:10.1101/pdb.rec10409 Cold Spring Harb Protoc 2006: pdb.rec10409-
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