NP-40 lysis buffer
For 1 liter of NP-40 lysis buffer, combine 30 ml of 5 M NaCl, 100 ml of 10% NP-40, 50 ml of 1 M Tris (pH 8.0), and 820 ml of H2O. Store at 4°C.
Note:
Triton X-100 can be used with similar results. Useful variations include lowering the detergent concentration, raising the salt concentration, or switching to other detergents such as saponin, digitonin, or CHAPS.
