Recipe

2X SDS-PAGE sample buffer

 
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4X stacking gel buffer

glycerol

10% SDS

2-mercaptoethanol

1.0% bromophenol blue

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Mix 2.0 ml of 4X stacking gel buffer, 1.6 ml of glycerol, 3.2 ml of 10% SDS, 0.8 ml of 2-mercaptoethanol , 0.4 ml of 1.0% bromophenol blue.

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  1. doi:10.1101/pdb.rec10476 Cold Spring Harb Protoc 2006: pdb.rec10476-
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