IPG Tris-HCl buffer (pH 8.8)
1.5 M Tris base
To make 25 ml, dissolve 4.55 g of Tris base and 0.1 g of SDS in ~20 ml of deionized H2O. Adjust the pH of the solution with 4 NHCl and adjust the volume to 25 ml with deionized H2O. Add 2.5 mg ofsodium azide and filter. The buffer can be stored at 4°C for up to 2 weeks.
