RNase Digestion Mixture

300 mM NaCl

10 mM Tris-Cl (pH 7.4)

5 mM EDTA (pH 7.5)

40 μg/ml RNase A

2 μg/ml RNase T1

Prepare 10 mg/ml ribonuclease A (bovine pancreatic RNase) in 10 mM Tris-Cl (pH 7.5), 15 mM NaCl. Prepare 1 mg/ml ribonuclease T1 in 10 mM Tris-Cl (pH 7.5), 15 mM NaCl.

Add the RNases fresh each time, just before digestion; 300 μl of digestion mixture is required for each digestion.