Recipe

MgCl2-CaCl2 solution (17-1)

 
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5 mM CaCl2

14 mM β-mercaptoethanol

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Sterilize the solution by filtration and store the filtrate at room temperature.

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  1. doi:10.1101/pdb.rec8084 Cold Spring Harb Protoc 2006: pdb.rec8084-
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