Complete Minimal (CM) or Synthetic Complete (SC) and Drop-out Media
Bact-yeast nitrogen base without amino acids*, 6.7 g
glucose, 20 g
Bacto Agar, 20 g
H2O, to 1000 ml
To test the growth requirements of strains, it is useful to have media in which each of the commonly encountered auxotrophies is supplemented except the one of interest (dropout media). Dry growth supplements are stored premixed.
CM (or SC) is a medium in which the drop-out mix contains all possible supplements (i.e., nothing is "dropped out").
*Yeast nitrogen base without amino acids (YNB) is sold either with or without ammonium sulfate. This recipe is for YNB with ammonium sulfate. If the bottle of YNB is lacking ammonium sulfate, add 5 g of ammonium sulfate and only 1.7 g of YNB.
