Ammonium persulfate
To prepare a 10% (w/v) solution: Dissolve 1 g ammonium persulfate in 10 mL of H2O and store at 4°C. Ammonium persulfate decays slowly in solution, so replace the stock solution every 2-3 wk. Ammonium persulfate is used as a catalyst for the copolymerization of acrylamide and bisacrylamide gels. The polymerization reaction is driven by free radicals generated by an oxido-reduction reaction in which a diamine (e.g., TEMED) is used as the adjunct catalyst.
