BLOTTO

5% (w/v) nonfat dried milk

0.02% sodium azide in H₂O

1x BLOTTO is 5% (w/v) nonfat dried milk dissolved in H₂O containing 0.02% sodium azide. 1x BLOTTO is stored at 4°C and is diluted 10-25-fold into prehybridization buffer before use. BLOTTO should not be used in combination with high concentrations of SDS, which will cause the milk proteins to precipitate. If background hybridization is a problem, Nonidet P-40 may be added to a final concentration of 1% (v/v).