Yeast selective X-gal medium
i. Prepare the appropriate CM base medium in 900 ml of H2O. Autoclave the base medium and cool it to 55°C.
ii. In a separate bottle, autoclave 7 g of sodium phosphate (dibasic) and 3 g of sodium phosphate (monobasic) in 100 ml of distilled H2O.
iii. Mix the two autoclaved solutions together, and add 0.8 ml of 100 mg/ml X-gal (in N,N-dimethylformamide). Pour the plates.
