Recipe

Yeast lysis solution

Zymolyase 100T dissolved to 2-5 mg/ml in rescue buffer

or

β-glucuronidase 100,000 units/ml (Sigma) 1:50 in rescue buffer

Prepare fresh solution for each use.

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  1. doi:10.1101/pdb.rec8845 Cold Spring Harb Protoc 2006: pdb.rec8845-
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