Recipe

EBC lysis buffer

 
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120 mM NaCl

0.5% (v/v) Nonidet P-40

5 μg/ml leupeptin

10 μg/ml aprotinin

50 μg/ml PMSF

0.2 mM sodium orthovanadate

100 mM NaF

50 mM Tris-Cl (pH 8.0)

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  1. doi:10.1101/pdb.rec8856 Cold Spring Harb Protoc 2006: pdb.rec8856-
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