Recipe

Mutagenesis buffer

 
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100 mM KCl

100 mM ammonium sulfate

200 mM Tris (pH 8.9 at room temperature)

20 mM MgSO4

1% Triton X-100

1 mg/ml nuclease-free bovine serum albumin

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For a 10x buffer. Mutagenesis buffers is used in place of Long PCR buffer with DNA polymerases such as Pfu.

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  1. doi:10.1101/pdb.rec9012 Cold Spring Harb Protoc 2006: pdb.rec9012-
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