Recipe

TNT buffer

 
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0.05% (v/v) Tween-20

10 mM Tris-Cl (pH 8.0)

150 mM NaCl

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Approximately 1 liter of TNT buffer is required per 10 filters screened. Store TNT buffer at room temperature.

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  1. doi:10.1101/pdb.rec9042 Cold Spring Harb Protoc 2006: pdb.rec9042-
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