Recipe

Lysis buffer (14-2)

100 mM Tris-Cl (pH 7.8)

1.5% (w/v) bovine serum albumin

1 μg/ml pancreatic DNase I

40 μg/ml lysozyme

150 mM NaCl

5 mM MgCl2

Add DNase I and lysozyme to this buffer just before use in Step 11.

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  1. doi:10.1101/pdb.rec9052 Cold Spring Harb Protoc 2006: pdb.rec9052-

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