Recipe

Cell resuspension buffer (14-4)

 
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10 mM EDTA (pH 8.0)

50 mM Tris-Cl (pH 8.0)

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Filter-sterilize and store the buffer at 4°C in 50-ml aliquots.

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  1. doi:10.1101/pdb.rec9057 Cold Spring Harb Protoc 2006: pdb.rec9057-
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