Recipe

Cell lysis buffer (14-5)

 
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0.1 M sodium borate (pH 8.0)

1 M NaCl

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Sterilize the cell lysis buffer using a 0.45-μm filter, and store at room temperature. Approximately 100 ml of cell lysis buffer is required per 1 liter of bacterial culture.

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  1. doi:10.1101/pdb.rec9059 Cold Spring Harb Protoc 2006: pdb.rec9059-
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