Recipe

Cell lysis buffer (14-9)

 
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50 mM Tris-Cl (pH 6.8)

2% (w/v) SDS

0.1% (w/v) bromophenol blue

10% (v/v) glycerol

100 mM dithiothreitol

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100 μl required for each plaque analyzed.

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  1. doi:10.1101/pdb.rec9076 Cold Spring Harb Protoc 2006: pdb.rec9076-
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