Recipe

Column regeneration buffer

 
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10 mM Tris-HCl (pH 7.6)

1 mM EDTA

2.5 M NaCl

1% Nonidet P-40 (NP-40)

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NP-40 will never completely dissolve. Swirl just before use.

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  1. doi:10.1101/pdb.rec9826 Cold Spring Harb Protoc 2006: pdb.rec9826-
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