Recipe

Universal restriction digestion buffer (10X)

 
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1 M potassium acetate

250 mM Tris-acetate, pH 7.6

100 mM magnesium acetate

100 μg/ml BSA

5mM β- mercaptoethanol

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  1. doi:10.1101/pdb.rec9866 Cold Spring Harb Protoc 2006: pdb.rec9866-
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