Recipe

Data from Amersham Biosciences (2003).

 
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Data from Amersham Biosciences (2003).

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Substance pKa Working pH Counterion
Formic acid 3.8 3.3- 4.3 NH+3
Acetate 4.7 4.3- 5.2 NH+4
Pyridine/acetate 5.35/4.74 4.3- 5.9 CH3COO−
N-Ethylmorpholine Ammonium 7.7 9.25 7.2- 8.2 8.7- 9.7 CH3COO− CH3COO−
Trimethylamine 9.8 9.3- 10.3 CH3COO−
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  1. doi:10.1101/pdb.rec9881 Cold Spring Harb Protoc 2006: pdb.rec9881-
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