Table 3. Ammonium sulfate saturation table using saturated ammonium sulfate solution

doi:10.1101/pdb.tab3

Table 3. Ammonium sulfate saturation table using saturated ammonium sulfate solution

Initial concentration of ammonium sulfate(% v/v)	Final concentration of ammonium sulfate(% v/v)
	10	15	20	25	30	35	40	45	50	55	60	65	70	75	80	85	90	95
	Milliliters saturated ammonium sulfate solution to add to 100 ml of solution^a,b
0	11	18	25	33	43	54	67	82	100	122	150	186	233	300	400	567	900	1900
5	6	12	19	26	36	46	58	73	90	111	138	171	217	280	375	533	850	1800
10	0	6	13	20	29	38	50	64	80	100	125	157	200	260	350	500	800	1700
15		0	6	13	21	31	42	55	70	89	113	143	183	240	325	466	750	1600

20			0	6	14	23	33	45	60	78	100	129	167	220	300	433	700	1500
25				0	7	15	25	36	50	67	88	114	150	200	275	400	650	1400
30					0	8	17	27	40	56	75	100	133	180	250	367	600	1300
35						0	8	18	30	44	63	86	117	160	225	333	550	1200
40							0	9	20	33	50	71	100	140	200	300	500	1100
45								0	10	22	38	57	83	120	175	267	450	1000

50									0	11	25	43	67	100	150	233	400	900
55										0	13	29	50	80	125	200	350	800
60											0	14	33	60	100	167	300	700
65												0	17	40	75	133	250	600
70													0	20	50	100	200	500

75														0	25	67	150	400
80															0	33	100	300
85																0	50	200
90																	0	100

Adapted from Scopes (1987).

Values given are the amount of saturated (NH₄)₂SO₄ solution required to bring a solution of known initial (NH₄)₂SO₄ concentration to a desired final (NH₄)₂SO₄ concentration. This table works equally well for determining the volume of a miscible solvent (such as ethanol or acetone) to be used for precipitation of proteins.

^aNote that the volume increases (sometimes significantly) as a result of addition of the precipitant.

^bThe values in this table are based on the following equation: Volume of saturated (NH₄)₂SO₄ to add to 100 ml = 100(C₂ - C₁)/(100 - C₂), where C₁ is the initial concentration of ammonium sulfate (% v/v) and C₂ is the final concentration of ammonium sulfate (% v/v).